SNAI1 is Involved in the Proliferation and Migration of Glioblastoma Cells

被引：97

作者：

Han, Sung-Pil ^{[1
,2
]}

Kim, Ji-Hoon ^{[1
]}

Han, Myoung-Eun ^{[2
]}

Sim, Hey-Eun ^{[1
,2
]}

Kim, Ki-Sun ^{[1
]}

Yoon, Sik ^{[1
]}

Baek, Sun-Yong ^{[1
]}

Kim, Bong-Seon ^{[1
]}

Oh, Sae-Ock ^{[1
,2
]}

机构：

[1] Pusan Natl Univ, Sch Med, Dept Anat, Yangsan 626870, South Korea

[2] Pusan Natl Univ, Med Res Ctr Ischem Tissue Regenerat, Yangsan 626870, South Korea

来源：

CELLULAR AND MOLECULAR NEUROBIOLOGY | 2011年 / 31卷 / 03期

关键词：

SNAI1; Glioblastoma; Proliferation; Migration; Invasion; EPITHELIAL-MESENCHYMAL TRANSITION; E-CADHERIN; HEPATOCELLULAR-CARCINOMA; CANCER CELLS; DIFFERENTIAL EXPRESSION; COLON-CANCER; SLUG; INVASION; GENE; INVASIVENESS;

D O I：

10.1007/s10571-010-9643-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Glioblastoma is the most common type of astrocytoma in the brain. Due to its high invasiveness and chemoresistance, patients with advanced stage of glioblastoma have a poor prognosis. SNAI1, an important regulator of epithelial-mesenchymal transition, has been associated with metastasis in various carcinoma cells. However, its roles in glioblastoma cells have been poorly characterized. To examine roles of SNAI1 in glioblastoma cells, we knockdowned SNAI1 expression using siRNA. SNAI1 siRNA increased the expression level of E-cadherin and decreased that of vimentin. In the water-soluble tetrazolium salt (WST-1) assay, SNAI1 siRNA inhibited the proliferation of U87-MG and GBM05 glioblastoma cells. Moreover, in the Boyden chamber assay and Matrigel invasion assay, SNAI1 siRNA inhibited serum-induced migration and invasion of glioblastoma cells. These results suggested that SNAI1 is involved in the proliferation and migration of glioblastoma cells.

引用

页码：489 / 496

页数：8

共 32 条

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