Control of neural stem cell differentiation on honeycomb films

被引：48
作者：
Tsuruma, Akinori
Tanaka, Masaru
Yamamoto, Sadaaki
Shimomura, Masatsugu
机构：
[1] Hokkaido Univ, Res Inst Elect Sci, Nanotechnol Res Ctr, Kita Ku, Sapporo, Hokkaido 0010021, Japan
[2] Hokkaido Univ, Creat Res Initiat Souseio, Kita Ku, Sapporo, Hokkaido 0010021, Japan
来源：
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2008年 / 313卷
关键词：
neuron; neural stem cell; neural regeneration; cell differentiation; micro and nano-pattern;
D O I：
10.1016/j.colsurfa.2007.05.079
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Control of neural stem cell (NSC) differentiation is ongoing interest in neural tissue engineering. Formation of neural networks on various patterned substrates was reported in previous studies. In this study, we cultured NSCs derived from the cerebral cortex of embryonic day-14 mice on honeycomb (HC) films with highly regular pores prepared by casting a polymer solution of water-immiscible solvent under high humidity. The differentiation of NSCs was analyzed by immunostaining for Nestin and MAP2. The differentiation of NSC was controlled for the first time by manipulating the pore size on HC films. The highest suppression of NSC differentiation was observed on HC film with 3 mu m pore specifically. (C) 2007 Elsevier B.V. All rights reserved.
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收藏
页码：536 / 540
页数：5
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