Production of functional lectin in Pichia pastoris directed by cloned cDNA from Aleuria aurantia

被引：13

作者：

Amano, K ^{[1
]}

Takase, M ^{[1
]}

Ando, A ^{[1
]}

Nagata, Y ^{[1
]}

机构：

[1] Chiba Univ, Fac Hort, Dept Bioresources Chem, Matsudo, Chiba 2718510, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2003年 / 67卷 / 10期

关键词：

Aleuria aurantia lectin; heterogenous gene expression; glycosylation of lectin; improvement of thermostability;

D O I：

10.1271/bbb.67.2277

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A plasmid bearing a nucleotide sequence of fucose-specific lectin of Aleuria aurantia was constructed and expressed in a methylotrophic yeast, Pichia pastoris. The product showed almost the same hemagglutinating activity as the lectin produced in Escherichia coli, the properties of which were quite similar to the native one. Because of glycosylation of the product, the molecular mass was larger than that of the native one, and it acquired higher thermostability.

引用

页码：2277 / 2279

页数：3

共 13 条

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