Stage-specific effects of Notch activation during skeletal myogenesis

被引:70
作者
Bi, Pengpeng [1 ]
Yue, Feng [1 ]
Sato, Yusuke [1 ]
Wirbisky, Sara [2 ]
Liu, Weiyi [1 ]
Shan, Tizhong [1 ]
Wen, Yefei [1 ]
Zhou, Daoguo [3 ]
Freeman, Jennifer [2 ,4 ]
Kuang, Shihuan [1 ,4 ]
机构
[1] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA
[2] Purdue Univ, Sch Hlth Sci, W Lafayette, IN 47907 USA
[3] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA
[4] Purdue Univ, Ctr Canc Res, W Lafayette, IN 47907 USA
来源
ELIFE | 2016年 / 5卷
关键词
DUCHENNE MUSCULAR-DYSTROPHY; QUIESCENT SATELLITE CELLS; MUSCLE PROGENITOR CELLS; STEM-CELLS; CARDIOMYOCYTE DEDIFFERENTIATION; MOLECULAR SIGNATURE; GENE-EXPRESSION; SELF-RENEWAL; REGENERATION; PAX7;
D O I
10.7554/eLife.17355
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Skeletal myogenesis involves sequential activation, proliferation, self-renewal/differentiation and fusion of myogenic stem cells (satellite cells). Notch signaling is known to be essential for the maintenance of satellite cells, but its function in late-stage myogenesis, i.e. post differentiation myocytes and post-fusion myotubes, is unknown. Using stage-specific Cre alleles, we uncovered distinct roles of Notchl in mononucleated myocytes and multinucleated myotubes. Specifically, constitutive Notchl activation dedifferentiates myocytes into Pax7 quiescent satellite cells, leading to severe defects in muscle growth and regeneration, and postnatal lethality. By contrast, myotube-specific Notchl activation improves the regeneration and exercise performance of aged and dystrophic muscles. Mechanistically, Notchl activation in myotubes upregulates the expression of Notch ligands, which modulate Notch signaling in the adjacent satellite cells to enhance their regenerative capacity. These results highlight context-dependent effects of Notch activation during myogenesis, and demonstrate that Notchl activity improves myotube's function as a stem cell niche.
引用
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页数:22
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