STRUCTURAL ANALYSIS OF RNA HELICASES WITH SMALL-ANGLE X-RAY SCATTERING

被引：5

作者：

Behrens, Manja A. ^{[1
,2
,3
]}

He, Yangzi ^{[3
,4
]}

Oliveira, Cristiano L. P. ^{[5
]}

Andersen, Gregers R. ^{[3
,4
]}

Pedersen, Jan Skov ^{[1
,2
,3
]}

Nielsen, Klaus H. ^{[3
,4
]}

机构：

[1] Aarhus Univ, Dept Chem, DK-8000 Aarhus, Denmark

[2] Aarhus Univ, iNANO Interdisciplinary Nanosci Ctr, Aarhus, Denmark

[3] Aarhus Univ, Ctr mRNP Biogenesis & Metab, Aarhus, Denmark

[4] Aarhus Univ, Dept Mol Biol & Genet, Aarhus, Denmark

[5] Univ Sao Paulo, Inst Fis, BR-01498 Sao Paulo, Brazil

来源：

RNA HELICASES | 2012年 / 511卷

关键词：

DIRECT SHAPE DETERMINATION; BIOLOGICAL MACROMOLECULES; CRYSTAL-STRUCTURE; RESOLUTION; INITIATION; PROTEINS; UNIQUENESS; COMPLEX; ATPASE; EIF4G;

D O I：

10.1016/B978-0-12-396546-2.00031-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Small-angle X-ray scattering (SAXS) is a structural characterization method applicable to biological macromolecules in solution. The great advantage of solution scattering is that the systems can be investigated in near-physiological conditions and their response to external changes can also be easily investigated. In this chapter, we discuss the application of SAXS for studying the conformation of helicases alone and in complex with other biological macromolecules. The DEAD-box helicase elF4A and the DEAH/RHA helicase Prp43 are investigated for their solution structures, and the analysis of the collected scattering data is presented. A wide range of methods for analysis of SAXS data are presented and discussed. Ab initio methods can be used to yield low-resolution solution structures, and when models with atomic resolution are available, these can be included to aid the determination of solution structures. Using such prior information relating to the systems studied and applying a variety of methods, substantial insight can be gained about solution structures and interactions of biological macromolecules through small-angle scattering.

引用

页码：191 / 212

页数：22

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