Carbonic anhydrases from leaves and roots of Daucus carota

被引：8

作者：

Demir, N ^{[1
]}

Demir, Y ^{[1
]}

Yildirim, A ^{[1
]}

机构：

[1] ATATURK UNIV, EGITIM FAKULTESI KIMYA EGITIMI BOLUMU, TR-25240 ERZURUM, TURKEY

来源：

PHYTOCHEMISTRY | 1997年 / 44卷 / 07期

关键词：

Daucus carota; Apraieae; wild carrot; carbonic anhydrase; caffeine; nicotine;

D O I：

10.1016/S0031-9422(96)00628-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Carbonic anhydrases (CAs) (carbonate hydrolase; EC 4.2.1.1) from leaves and roots of mature Daucus carota cv. nantes were purified and characterized. The purification levels were 75- and 73-fold in leaves and roots, respectively. The optimum temperature for the enzymes from both sources was 75 degrees. pH optima were 8.5 and 9.0 in leaves and in roots, respectively. Each enzyme molecule was a hexamer having M(r) 137 800 and the subunit M(r) was 22 800. A dimer CA which has a M(r) of 45 700 was also detected. The amount of this dimer was higher in leaves than in roots. Copyright (C) 1997 Elsevier Science Ltd.

引用

页码：1247 / 1250

页数：4

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