High-Throughput Cryo-EM Enabled by User-Free Preprocessing Routines

被引:33
|
作者
Li, Yilai [1 ]
Cash, Jennifer N. [1 ]
Tesmer, John J. G. [2 ,3 ]
Cianfrocco, Michael A. [1 ]
机构
[1] Univ Michigan, Dept Biol Chem, Life Sci Inst, Ann Arbor, MI 48109 USA
[2] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA
[3] Purdue Univ, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA
关键词
MICROSCOPY;
D O I
10.1016/j.str.2020.03.008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Single-particle cryoelectron microscopy (cryo-EM) continues to grow into a mainstream structural biology technique. Recent developments in data collection strategies alongside new sample preparation devices herald a future where users will collect multiple datasets per microscope session. To make cryo-EM data processing more automatic and user-friendly, we have developed an automatic pipeline for cryo-EM data preprocessing and assessment using a combination of deep-learning and image-analysis tools. We have verified the performance of this pipeline on a number of datasets and extended its scope to include sample screening by the user-free assessment of the qualities of a series of datasets under different conditions. We propose that our workflow provides a decision-free solution for cryo-EM, making data preprocessing more generalized and robust in the high-throughput era as well as more convenient for users from a range of backgrounds.
引用
收藏
页码:858 / +
页数:15
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