Gallic acid-loaded electrospun cellulose acetate nanofibers as potential wound dressing materials

Gallic acid (GA)-loaded cellulose acetate (CA) nanofiber mats with 10 to 40 wt.% GA contents (based on the weight of CA) were fabricated by electrospinning. The effects of GA contents and applied potential on the morphology and the average diameters of fibers were studied. The electrospun fiber mats containing 20 and 40 wt.% GA were investigated for their potential use as carrier of GA in wound dressing application. The GA-loaded CA films were prepared by solvent casting technique for use in comparative studies. Determination of the release characteristics of GA from the GA-loaded fiber mats and films was carried out by the total immersion and the transdermal diffusion through a pig skin method in acetate buffer solution (pH 5.5) or normal saline (pH 7.0) at either 32 or 37 degrees C, respectively. In the total immersion method, the maximum amounts of the GA released from the fiber mats containing 20 and 40 wt.% GA in the acetate buffer were approximately 97% and 71% (based on the weight of initial GA), while those of the GA released into the normal saline were approximately 96% and 81%, respectively. Lower values were observed in the experiments of the transdermal diffusion through a pig skin method. The corresponding GA-loaded CA films showed the lower amounts of GA released into media. The as-loaded and the as-released GA remained its antioxidant activity as investigated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Lastly, the GA-loaded CA fiber mats exhibited antibacterial activity against Staphylococcus aureus, which showed the potential for use as wound dressing materials.