Is Aeromonas hydrophila the dominant motile Aeromonas species that causes disease outbreaks in aquaculture production in the Zhejiang Province of China?

被引:199
作者
Nielsen, ME
Hoi, L
Schmidt, AS
Qian, D
Shimada, T
Shen, JY
Larsen, JL
机构
[1] Royal Vet & Agr Univ, Dept Vet Microbiol, DK-1870 Frederiksberg C, Denmark
[2] Zhejiang Inst Freshwater Fisheries, Huzhou 313001, Zhejiang, Peoples R China
[3] Natl Inst Infect Dis, Tokyo 1628640, Japan
关键词
Aeromonas hydrophila; PCR; China; haemorrhagic septicaemia; fish diseases;
D O I
10.3354/dao046023
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
The significance of Aeromonas hydrophila in association with disease outbreaks in aquaculture production in the Zhejiang province of China was investigated. Bacteriological examination of moribund fish and crabs resulted in 95 bacterial isolates: 88 bacterial isolates from fish and 7 isolates from crabs. PCR and traditional biochemical methods were used for identification of A. hydrophila. Out of 69 motile aeromonads, 35 isolates were identified as A. hydrophila by biochemical tests. However, 6 of those were not identified as A. hydrophila by a species specific PCR method. Serotyping revealed 2 dominant serotypes (09 and 097) among A. hydrophila isolates. The data presented show that approximately 42% of the motile aeromonads isolated from disease outbreaks among various fish species were A. hydrophila. It is noteworthy that A. hydrophila accounted for more than 50% of the isolated aeromonands isolated from crucian carp Carassius carassius and Wuchang bream Megalobrama amblycephala with haemorrhagic septicaemia. Although this species was the most frequently isolated organism from internal organs of diseased fish and crabs in the present study, other motile Aeromonas spp. were also found. The PCR assay was useful in preventing misidentification of A. hydrophila, which may occur when only phenotypic tests are employed.
引用
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页码:23 / 29
页数:7
相关论文
共 24 条
[1]  
AUSTIN B, 1996, GENUS AEROMONAS, P197
[2]  
Barrow GI., 1993, COWAN STEELS MANUAL, V3th
[3]   RAPID IDENTIFICATION OF AEROMONAS SPECIES USING 16S RDNA TARGETED OLIGONUCLEOTIDE PRIMERS - A MOLECULAR APPROACH BASED ON SCREENING OF ENVIRONMENTAL ISOLATES [J].
DORSCH, M ;
ASHBOLT, NJ ;
COX, PT ;
GOODMAN, AE .
JOURNAL OF APPLIED BACTERIOLOGY, 1994, 77 (06) :722-726
[4]   AEROMONAS ENCHELEIA SP-NOV, ISOLATED FROM EUROPEAN EELS [J].
ESTEVE, C ;
GUTIERREZ, MC ;
VENTOSA, A .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1995, 45 (03) :462-466
[5]  
*FAO, 1997, 815 FAO FISH DEP
[6]  
*FAO, 1998, FISH DEP REP STAT WO
[7]  
Haenninen M. L., 1994, Journal of Applied Bacteriology, V76, P455
[8]   MORPHOLOGICAL HETEROGENEITY AMONG SALMONELLA LIPOPOLYSACCHARIDE CHEMOTYPES IN SILVER-STAINED POLYACRYLAMIDE GELS [J].
HITCHCOCK, PJ ;
BROWN, TM .
JOURNAL OF BACTERIOLOGY, 1983, 154 (01) :269-277
[9]  
Holt G.J., 1994, Bergey's Manual of Determinative Bacteriology
[10]  
IQUBAL MM, 1998, B FAC FISH HOKKAIDO, V49, P131