Improved Biosurfactant Production by Bacillus subtilis SPB1 Mutant Obtained by Random Mutagenesis and Its Application in Enhanced Oil Recovery in a Sand System

被引:41
作者
Bouassida, Mouna [1 ]
Ghazala, Imen [2 ,3 ]
Ellouze-Chaabouni, Semia [1 ,4 ]
Ghribi, Dhouha [1 ,5 ]
机构
[1] Univ Sfax, Ecole Natl Ingn Sfax, Unite Enzymes & Bioconvers, Sfax, Tunisia
[2] Ecole Natl Ingn Sfax, Lab Ameliorat Plantes & Valorisat Agroressources, Sfax, Tunisia
[3] Univ Sfax, Sfax, Tunisia
[4] Univ Sfax, Ecole Natl Ingn Sfax, Unite Serv Commun Bioreacteur Couple Ultrafiltre, Sfax, Tunisia
[5] Univ Sfax, Inst Super Biotechnol Sfax, Sfax, Tunisia
关键词
Random mutagenesis; biosurfactant; Bacillus subtilis; fermentation; oil recovery; ULTRAVIOLET-LIGHT; CONTAMINATED SOIL; STABILITY; THURINGIENSIS; SELECTION; IDENTIFICATION; OPTIMIZATION; IRRADIATION; SOLUBILITY; SURFACTANT;
D O I
10.4014/jmb.1701.01033
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Biosurfactants or microbial surfactants are surface-active biomolecules that are produced by a variety of microorganisms. Biodegradability and low toxicity have led to the intensification of scientific studies on a wide range of industrial applications for biosurfactants in the field of environmental bioremediation as well as the petroleum industry and enhanced oil recovery. However, the major issues in biosurfactant production are high production cost and low yield. Improving the bioindustrial production processes relies on many strategies, such as the use of cheap raw materials, the optimization of medium-culture conditions, and selecting hyperproducing strains. The present work aims to obtain a mutant with higher biosurfactant production through applying mutagenesis on Bacillus subtilis SPB1 using a combination of UV irradiation and nitrous acid treatment. Following mutagenesis and screening on blood agar and subsequent formation of halos, the mutated strains were examined for emulsifying activity of their culture broth. A mutant designated B. subtilis M2 was selected as it produced biosurfactant at twice higher concentration than the parent strain. The potential of this biosurfactant for industrial uses was shown by studying its stability to environmental stresses such as pH and temperature and its applicability in the oil recovery process. It was practically stable at high temperature and at a wide range of pH, and it recovered above 90% of motor oil adsorbed to a sand sample.
引用
收藏
页码:95 / 104
页数:10
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