Facile synthesis of fluorescent tungsten oxide quantum dots for telomerase detection based on the inner filter effect

被引:5
|
作者
Zhang, Li [1 ]
Chen, Jia-Qing [1 ]
Hong, Ming-Fang [1 ]
Liang, Ru-Ping [1 ]
Qiu, Jian-Ding [1 ,2 ]
机构
[1] Nanchang Univ, Coll Chem, Nanchang 330031, Jiangxi, Peoples R China
[2] Pingxiang Univ, Environm Protect Mat & Equipment Engn Technol Ctr, Dept Mat & Chem Engn, 211 Pingan North Rd, Pingxiang 337055, Jiangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
NANOPARTICLES; EFFICIENT; CATALYST; DNA;
D O I
10.1039/d0an00296h
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The traditional detection of telomerase activity is mainly based on the polymerase chain reaction (PCR), which has the disadvantages of being time-consuming and susceptible to interferences; thus, here, we propose a facile method for the fabrication of fluorescent tungsten oxide quantum dots (WOx QDs) and employ them for telomerase activity sensing. It is found that the fluorescence of WOx QDs can be significantly quenched by hemin based on the inner filter effect (IFE). However, in the presence of telomerase, the primer-DNA can be extended to generate repeating units of TTAGGG to form G-quadruplex and thus, hemin can be encapsulated to reduce its absorbance, resulting in decreased IFE and efficient fluorescence recovery of WOx QDs. Based on the fluorescence changes of IFE between hemin and WOx QDs, the telomerase activity within the range of 50-30 000 HeLa cells can be detected and the lowest detection amount can reach 17 cells. The method exhibits good versatility that can also be applied to telomerase detection in A549 and L929 cells. In addition, because of the good biocompatibility of the sensor, it can be used for the real-time monitoring of telomerase activity in living cells, thus showing great potential in tumor diagnosis and inhibitor drug screening.
引用
收藏
页码:2570 / 2579
页数:10
相关论文
共 50 条
  • [31] Black Phosphorus Quantum Dots as the Ratiometric Fluorescence Probe for Trace Mercury Ion Detection Based on Inner Filter Effect
    Gu, Wei
    Pei, Xueyu
    Cheng, Yuxiao
    Zhang, Cuiling
    Zhang, Jidong
    Yan, Yinghan
    Ding, Caiping
    Xian, Yuezhong
    ACS SENSORS, 2017, 2 (04): : 576 - 582
  • [32] Facile synthesis of surface-functionalized fluorescent carbon quantum dots for the selective detection of ferric ions
    Hijazi, Shariqah
    Ganie, Adil Shafi
    Rahman, Mohammed M.
    Shah, Wajaht A.
    ENVIRONMENTAL SCIENCE-NANO, 2023, 10 (12) : 3281 - 3294
  • [33] Carbon quantum dot-AgOH colloid fluorescent probe for selective detection of biothiols based on the inner filter effect
    Zhou, Nan
    Shi, Yanping
    Sun, Chao
    Zhang, Xingwei
    Zhao, Wei
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2020, 228
  • [34] Highly fluorescent nitrogen and sulfur co-doped graphene quantum dots for an inner filter effect-based cyanide sensor
    Chen, Chuanxia
    Zhao, Dan
    Hu, Tao
    Sun, Jian
    Yang, Xiurong
    SENSORS AND ACTUATORS B-CHEMICAL, 2017, 241 : 779 - 788
  • [35] Aptamer-based fluorescent screening assay for acetamiprid via inner filter effect of gold nanoparticles on the fluorescence of CdTe quantum dots
    Jiajia Guo
    Ying Li
    Luokai Wang
    Jingyue Xu
    Yanjun Huang
    Yeli Luo
    Fei Shen
    Chunyan Sun
    Rizeng Meng
    Analytical and Bioanalytical Chemistry, 2016, 408 : 557 - 566
  • [36] Aptamer-based fluorescent screening assay for acetamiprid via inner filter effect of gold nanoparticles on the fluorescence of CdTe quantum dots
    Guo, Jiajia
    Li, Ying
    Wang, Luokai
    Xu, Jingyue
    Huang, Yanjun
    Luo, Yeli
    Shen, Fei
    Sun, Chunyan
    Meng, Rizeng
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2016, 408 (02) : 557 - 566
  • [37] A facile ratiometric sensing platform based on inner filter effect for hypochlorous acid detection
    Xu, Chenggong
    Zhou, Yanmei
    Zhou, Yunhao
    Li, Zhaoge
    Peng, Xiaojun
    SENSORS AND ACTUATORS B-CHEMICAL, 2020, 325
  • [38] Functionalized carbon dots of thiazole derivatives based on inner filter effect for tetracyclines detection
    Yan, Fanyong
    Sun, Zhonghui
    Pang, Jiping
    Jiang, Yingxia
    Zheng, Wenxuan
    DYES AND PIGMENTS, 2020, 183 (183)
  • [39] Facile synthesis of highly efficient fluorescent carbon dots for tetracycline detection
    Guo, Feng
    Zhu, Zihan
    Zheng, Zhangqin
    Jin, Ying
    Di, Xiaoxuan
    Xu, Zhonghao
    Guan, Hongwei
    ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2020, 27 (04) : 4520 - 4527
  • [40] Facile synthesis of highly efficient fluorescent carbon dots for tetracycline detection
    Feng Guo
    Zihan Zhu
    Zhangqin Zheng
    Ying Jin
    Xiaoxuan Di
    Zhonghao Xu
    Hongwei Guan
    Environmental Science and Pollution Research, 2020, 27 : 4520 - 4527