Facile synthesis of fluorescent tungsten oxide quantum dots for telomerase detection based on the inner filter effect

被引:5
|
作者
Zhang, Li [1 ]
Chen, Jia-Qing [1 ]
Hong, Ming-Fang [1 ]
Liang, Ru-Ping [1 ]
Qiu, Jian-Ding [1 ,2 ]
机构
[1] Nanchang Univ, Coll Chem, Nanchang 330031, Jiangxi, Peoples R China
[2] Pingxiang Univ, Environm Protect Mat & Equipment Engn Technol Ctr, Dept Mat & Chem Engn, 211 Pingan North Rd, Pingxiang 337055, Jiangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
NANOPARTICLES; EFFICIENT; CATALYST; DNA;
D O I
10.1039/d0an00296h
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The traditional detection of telomerase activity is mainly based on the polymerase chain reaction (PCR), which has the disadvantages of being time-consuming and susceptible to interferences; thus, here, we propose a facile method for the fabrication of fluorescent tungsten oxide quantum dots (WOx QDs) and employ them for telomerase activity sensing. It is found that the fluorescence of WOx QDs can be significantly quenched by hemin based on the inner filter effect (IFE). However, in the presence of telomerase, the primer-DNA can be extended to generate repeating units of TTAGGG to form G-quadruplex and thus, hemin can be encapsulated to reduce its absorbance, resulting in decreased IFE and efficient fluorescence recovery of WOx QDs. Based on the fluorescence changes of IFE between hemin and WOx QDs, the telomerase activity within the range of 50-30 000 HeLa cells can be detected and the lowest detection amount can reach 17 cells. The method exhibits good versatility that can also be applied to telomerase detection in A549 and L929 cells. In addition, because of the good biocompatibility of the sensor, it can be used for the real-time monitoring of telomerase activity in living cells, thus showing great potential in tumor diagnosis and inhibitor drug screening.
引用
收藏
页码:2570 / 2579
页数:10
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