Reducing the Oxidation Level of Dextran Aldehyde in a Chitosan/Dextran-Based Surgical Hydrogel Increases Biocompatibility and Decreases Antimicrobial Efficacy
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作者:
Chan, Maggie
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Univ Otago, Dept Microbiol & Immunol, Dunedin 9054, New ZealandUniv Otago, Dept Microbiol & Immunol, Dunedin 9054, New Zealand
Chan, Maggie
[1
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Brooks, Heather J. L.
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Univ Otago, Dept Microbiol & Immunol, Dunedin 9054, New ZealandUniv Otago, Dept Microbiol & Immunol, Dunedin 9054, New Zealand
Brooks, Heather J. L.
[1
]
Moratti, Stephen C.
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Univ Otago, Dept Chem, Dunedin 9054, New ZealandUniv Otago, Dept Microbiol & Immunol, Dunedin 9054, New Zealand
Moratti, Stephen C.
[2
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Hanton, Lyall R.
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Univ Otago, Dept Chem, Dunedin 9054, New ZealandUniv Otago, Dept Microbiol & Immunol, Dunedin 9054, New Zealand
Hanton, Lyall R.
[2
]
Cabral, Jaydee D.
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Univ Otago, Dept Chem, Dunedin 9054, New ZealandUniv Otago, Dept Microbiol & Immunol, Dunedin 9054, New Zealand
A highly oxidized form of a chitosan/dextran-based hydrogel (CD-100) containing 80% oxidized dextran aldehyde (DA-100) was developed as a post-operative aid, and found to significantly prevent adhesion formation in endoscopic sinus surgery (ESS). However, the CD-100 hydrogel showed moderate in vitro cytotoxicity to mammalian cell lines, with the DA-100 found to be the cytotoxic component. In order to extend the use of the hydrogel to abdominal surgeries, reformulation using a lower oxidized DA (DA-25) was pursued. The aim of the present study was to compare the antimicrobial efficacy, in vitro biocompatibility and wound healing capacity of the highly oxidized CD-100 hydrogel with the CD-25 hydrogel. Antimicrobial studies were performed against a range of clinically relevant abdominal microorganisms using the micro-broth dilution method. Biocompatibility testing using human dermal fibroblasts was assessed via a tetrazolium reduction assay (MTT) and a wound healing model. In contrast to the original DA-100 formulation, DA-25 was found to be non-cytotoxic, and showed no overall impairment of cell migration, with wound closure occurring at 72 h. However, the lower oxidation level negatively affected the antimicrobial efficacy of the hydrogel (CD-25). Although the CD-25 hydrogel's antimicrobial efficacy and anti-fibroblast activity is decreased when compared to the original CD-100 hydrogel formulation, previous in vivo studies show that the CD-25 hydrogel remains an effective, biocompatible barrier agent in the prevention of postoperative adhesions.
机构:
Univ Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, AustraliaUniv Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, Australia
Athanasiadis, Theo
;
beule, Achim G.
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Ernst Moritz Arndt Univ Greifswald, Dept Otorhinolaryngol Head & Neck Surg, Greifswald, GermanyUniv Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, Australia
beule, Achim G.
;
Robinson, Brian H.
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Univ Otago, Dept Chem, Dunedin, New ZealandUniv Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, Australia
Robinson, Brian H.
;
Robinson, Simon R.
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Wakefield Hosp, Sinus & Facial Plast Ctr, Wellington, New ZealandUniv Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, Australia
Robinson, Simon R.
;
Shi, Z.
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Univ Otago, Dept Chem, Dunedin, New ZealandUniv Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, Australia
Shi, Z.
;
Wormald, Peter-John
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机构:
Univ Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, AustraliaUniv Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, Australia
机构:
Univ Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, AustraliaUniv Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, Australia
Athanasiadis, Theo
;
beule, Achim G.
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h-index: 0
机构:
Ernst Moritz Arndt Univ Greifswald, Dept Otorhinolaryngol Head & Neck Surg, Greifswald, GermanyUniv Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, Australia
beule, Achim G.
;
Robinson, Brian H.
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机构:
Univ Otago, Dept Chem, Dunedin, New ZealandUniv Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, Australia
Robinson, Brian H.
;
Robinson, Simon R.
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机构:
Wakefield Hosp, Sinus & Facial Plast Ctr, Wellington, New ZealandUniv Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, Australia
Robinson, Simon R.
;
Shi, Z.
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h-index: 0
机构:
Univ Otago, Dept Chem, Dunedin, New ZealandUniv Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, Australia
Shi, Z.
;
Wormald, Peter-John
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机构:
Univ Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, AustraliaUniv Adelaide, Dept Otorhinolaryngol Head & Neck Surg, Adelaide, SA, Australia