Reducing the Oxidation Level of Dextran Aldehyde in a Chitosan/Dextran-Based Surgical Hydrogel Increases Biocompatibility and Decreases Antimicrobial Efficacy

被引:26
作者
Chan, Maggie [1 ]
Brooks, Heather J. L. [1 ]
Moratti, Stephen C. [2 ]
Hanton, Lyall R. [2 ]
Cabral, Jaydee D. [2 ]
机构
[1] Univ Otago, Dept Microbiol & Immunol, Dunedin 9054, New Zealand
[2] Univ Otago, Dept Chem, Dunedin 9054, New Zealand
关键词
PREVENT PERITONEAL ADHESIONS; ENDOSCOPIC SINUS SURGERY; CONTROLLED-TRIAL; SHEEP MODEL; GEL; BARRIER; HEMOSTASIS; INFECTIONS; SAFETY;
D O I
10.3390/ijms160613798
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A highly oxidized form of a chitosan/dextran-based hydrogel (CD-100) containing 80% oxidized dextran aldehyde (DA-100) was developed as a post-operative aid, and found to significantly prevent adhesion formation in endoscopic sinus surgery (ESS). However, the CD-100 hydrogel showed moderate in vitro cytotoxicity to mammalian cell lines, with the DA-100 found to be the cytotoxic component. In order to extend the use of the hydrogel to abdominal surgeries, reformulation using a lower oxidized DA (DA-25) was pursued. The aim of the present study was to compare the antimicrobial efficacy, in vitro biocompatibility and wound healing capacity of the highly oxidized CD-100 hydrogel with the CD-25 hydrogel. Antimicrobial studies were performed against a range of clinically relevant abdominal microorganisms using the micro-broth dilution method. Biocompatibility testing using human dermal fibroblasts was assessed via a tetrazolium reduction assay (MTT) and a wound healing model. In contrast to the original DA-100 formulation, DA-25 was found to be non-cytotoxic, and showed no overall impairment of cell migration, with wound closure occurring at 72 h. However, the lower oxidation level negatively affected the antimicrobial efficacy of the hydrogel (CD-25). Although the CD-25 hydrogel's antimicrobial efficacy and anti-fibroblast activity is decreased when compared to the original CD-100 hydrogel formulation, previous in vivo studies show that the CD-25 hydrogel remains an effective, biocompatible barrier agent in the prevention of postoperative adhesions.
引用
收藏
页码:13798 / 13814
页数:17
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