Preparative Separation of Three Monoterpenes from Perilla frutescens var. crispa Using Centrifugal Partition Chromatography

被引:5
作者
Nam, Bomi [1 ]
Paudel, Sunil Babu [2 ]
Kim, Jin-Baek [1 ]
Jin, Chang Hyun [1 ]
Lee, Dongho [3 ]
Nam, Joo-Won [2 ]
Han, Ah-Reum [1 ]
机构
[1] Korea Atom Energy Res Inst, Adv Radiat Technol Inst, Jeongeup Si 56212, Jeollabuk Do, South Korea
[2] Yeungnam Univ, Coll Pharm, Gyongsan 38541, Gyeongsangbuk D, South Korea
[3] Korea Univ, Dept Biosyst & Biotechnol, Seoul 02841, South Korea
基金
新加坡国家研究基金会;
关键词
NITRIC-OXIDE PRODUCTION; HEME OXYGENASE-1; NATURAL-PRODUCTS; INDUCTION; EXTRACT; CELLS;
D O I
10.1155/2019/8751345
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Three monoterpenes, namely, 9-hydroxy isoegomaketone (1), isoegomaketone (2), and perilla ketone (3), were successfully separated from the supercritical carbon dioxide (SC-CO2) extract of the leaves of Perilla frutescens var. crispa (cv. Antisperill; Lamiaceae) by centrifugal partition chromatography (CPC). To obtain large quantities of these materials required for studies on their mechanism of action and in vivo effectiveness in inflammation, we used CPC because of its high loading capacity and reproducibility to purify the three compounds. Compound 1 (2.60mg, 96.7% purity at 254nm) was purified from 500mg of the SC-CO2 extract of P. frutescens var. crispa (cv. Antisperill), using a two-phase solvent system comprising n-hexane/ethyl acetate/ethanol/water (5:5:5:5v/v) in a descending mode. As compounds 2 (56.1mg, 97.6% purity at 254nm) and 3 (78.6mg, 96.1% purity at 254nm) are highly volatile and difficult to recover from an aqueous mobile phase after purification during the drying process, they were obtained from the same amount of the processed extract in an ascending mode using the upper organic phase as the mobile phase (n-hexane/ethyl acetate/ethanol/water, 8:2:8:2v/v). The structures of compounds 1-3 were confirmed by H-1- and C-13-NMR analysis. Thus, based on our findings, we recommend centrifugal partition chromatography as a powerful technique for purifying the active principal compounds 1 and 2 from the leaves of P. frutescens var. crispa.
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页数:7
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