2D protein electrophoresis: can it be perfected?

被引：131
作者：
Celis, JE
Gromov, P
机构：
[1] Aarhus Univ, Dept Biochem Med, DK-8000 Aarhus C, Denmark
[2] Aarhus Univ, Danish Ctr Human Genome Res, DK-8000 Aarhus C, Denmark
来源：
CURRENT OPINION IN BIOTECHNOLOGY | 1999年 / 10卷 / 01期
关键词：
D O I：
10.1016/S0958-1669(99)80004-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
23 years after O'Farrel developed two-dimensional gel electrophoresis we still debate if the technique can be improved, or if there are other alternative separation technologies that can challenge its central position in proteomic projects. These questions are relevant as the pharmaceutical industry expects proteomic studies to provide novel protein targets for drug discovery and diagnostics. In our opinion, there are various aspects of the technology that can be improved, including resolution, sample preparation and detection, but so far there is no alternative technique(s) available, or any under development, that can replace it.
引用
收藏
页码：16 / 21
页数：6
相关论文
共 39 条
  • [1] AEBERSOLD RH, 1986, J BIOL CHEM, V261, P4229
  • [2] Proteome and proteomics: New technologies, new concepts, and new words
    Anderson, NL
    Anderson, NG
    [J]. ELECTROPHORESIS, 1998, 19 (11) : 1853 - 1861
  • [3] PROTEIN-ELECTROBLOTTING AND PROTEIN-MICROSEQUENCING STRATEGIES IN GENERATING PROTEIN DATA-BASES FROM TWO-DIMENSIONAL GELS - (COMPUTERIZED PROTEIN DATA-BASES HUMAN GENOME SEQUENCING)
    BAUW, G
    VANDAMME, J
    PUYPE, M
    VANDEKERCKHOVE, J
    GESSER, B
    RATZ, GP
    LAURIDSEN, JB
    CELIS, JE
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (20) : 7701 - 7705
  • [4] ISOELECTRIC-FOCUSING IN IMMOBILIZED PH GRADIENTS - PRINCIPLE, METHODOLOGY AND SOME APPLICATIONS
    BJELLQVIST, B
    EK, K
    RIGHETTI, PG
    GIANAZZA, E
    GORG, A
    WESTERMEIER, R
    POSTEL, W
    [J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1982, 6 (04): : 317 - 339
  • [5] REFERENCE POINTS FOR COMPARISONS OF 2-DIMENSIONAL MAPS OF PROTEINS FROM DIFFERENT HUMAN CELL-TYPES DEFINED IN A PH SCALE WHERE ISOELECTRIC POINTS CORRELATE WITH POLYPEPTIDE COMPOSITIONS
    BJELLQVIST, B
    BASSE, B
    OLSEN, E
    CELIS, JE
    [J]. ELECTROPHORESIS, 1994, 15 (3-4) : 529 - 539
  • [6] Proteomics: key technology in drug discovery
    Celis, JE
    [J]. DRUG DISCOVERY TODAY, 1998, 3 (05) : 193 - 195
  • [7] Human and mouse proteomic databases: novel resources in the protein universe
    Celis, JE
    Ostergaard, M
    Jensen, NA
    Gromova, I
    Rasmussen, HH
    Gromov, P
    [J]. FEBS LETTERS, 1998, 430 (1-2): : 64 - 72
  • [8] The human keratinocyte two-dimensional gel protein database (update 1995): Mapping components of signal transduction pathways
    Celis, JE
    Rasmussen, HH
    Gromov, P
    Olsen, E
    Madsen, P
    Leffers, H
    Honore, B
    Dejgaard, K
    Vorum, H
    Kristensen, DB
    Ostergaard, M
    Haunso, A
    Jensen, NA
    Celis, A
    Basse, B
    Lauridsen, JB
    Ratz, GP
    Andersen, AH
    Walbum, E
    Kjaergaard, I
    Andersen, I
    Puype, M
    VanDamme, J
    Vandekerckhove, J
    [J]. ELECTROPHORESIS, 1995, 16 (12) : 2177 - +
  • [9] Celis JE, 1998, CELL BIOLOGY - A LABORATORY HANDBOOK, 2ND EDITION, VOL 2, P429
  • [10] HUMAN CELLULAR PROTEIN-PATTERNS AND THEIR LINK TO GENOME DNA-SEQUENCE DATA - USEFULNESS OF 2-DIMENSIONAL GEL-ELECTROPHORESIS AND MICROSEQUENCING
    CELIS, JE
    RASMUSSEN, HH
    LEFFERS, H
    MADSEN, P
    HONORE, B
    GESSER, B
    DEJGAARD, K
    VANDEKERCKHOVE, J
    [J]. FASEB JOURNAL, 1991, 5 (08) : 2200 - 2208
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