2D protein electrophoresis: can it be perfected?

被引：133

作者：

Celis, JE

Gromov, P

机构：

[1] Aarhus Univ, Dept Biochem Med, DK-8000 Aarhus C, Denmark

[2] Aarhus Univ, Danish Ctr Human Genome Res, DK-8000 Aarhus C, Denmark

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1999年 / 10卷 / 01期

关键词：

D O I：

10.1016/S0958-1669(99)80004-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

23 years after O'Farrel developed two-dimensional gel electrophoresis we still debate if the technique can be improved, or if there are other alternative separation technologies that can challenge its central position in proteomic projects. These questions are relevant as the pharmaceutical industry expects proteomic studies to provide novel protein targets for drug discovery and diagnostics. In our opinion, there are various aspects of the technology that can be improved, including resolution, sample preparation and detection, but so far there is no alternative technique(s) available, or any under development, that can replace it.

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页码：16 / 21

页数：6

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