Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy

被引：0

作者：

Bothe, Jameson R. ^{[1
]}

Nikolova, Evgenia N. ^{[2
]}

Eichhorn, Catherine D. ^{[2
]}

Chugh, Jeetender ^{[3
]}

Hansen, Alexandar L. ^{[4
,5
,6
]}

Al-Hashimi, Hashim M. ^{[1
,3
]}

机构：

[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Chem Biol Doctoral Program, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Dept Biophys, Ann Arbor, MI 48109 USA

[4] Univ Toronto, Dept Chem, Toronto, ON M5S 1A1, Canada

[5] Univ Toronto, Dept Biochem, Toronto, ON M5S 1A1, Canada

[6] Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A1, Canada

来源：

NATURE METHODS | 2011年 / 8卷 / 11期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

RESIDUAL DIPOLAR COUPLINGS; IMINO PROTON-EXCHANGE; BASE-PAIR KINETICS; LIQUID-CRYSTALLINE MEDIUM; INTRAMOLECULAR STEM-LOOP; CHEMICAL-SHIFT TENSORS; MODEL-FREE APPROACH; REAL-TIME NMR; SPIN RELAXATION; NUCLEIC-ACIDS;

D O I：

10.1038/NMETH.1735

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Many recently discovered noncoding RNAs do not fold into a single native conformation but sample many different conformations along their free-energy landscape to carry out their biological function. Here we review solution-state NMR techniques that measure the structural, kinetic and thermodynamic characteristics of RNA motions spanning picosecond to second timescales at atomic resolution, allowing unprecedented insights into the RNA dynamic structure landscape. From these studies a basic description of the RNA dynamic structure landscape is emerging, bringing new insights into how RNA structures change to carry out their function as well as applications in RNA-targeted drug discovery and RNA bioengineering.

引用

页码：919 / 931

页数：13

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