Sensitive determination of aluminum in parenteral solutions and injections by HPLC with fluorescence detection using lumogallion

A sensitive fluorometric method for the determination of aluminum has been developed using lumogallion [5-Chloro-2-hydroxy-3-(2,4-dihydroxy phenylazo) benzenesulfonic acid]. Aluminum is known to form a fluorescent chelate with lumogallion. The aluminum-lumogallion complex was separated on a reversed-phase column within 6 minutes and detected by a fluorescence detector at 505 nm (excitation wavelength) and 574 nm (emission wavelength). The present method enabled detection down to 0.1 mug/l in an aqueous solution, and the relative standard deviation (RSD) at 50 mug/l of aluminum was less than 1%. We applied it to the determination of aluminum in not only parenteral solutions and injections including a matrix, such as vitamins, glucose and lipid, but also to amino acids as their ingredients. The recovery of this method was, generally over 90%.