MicroRNA-21 attenuates oxygen and glucose deprivation induced apoptotic death in human neural stem cells with inhibition of JNK and p38 MAPK signaling

被引:13
作者
Chen, Rui [1 ]
Tai, Yingchun [2 ]
Zhang, Yurong [3 ]
Wang, Li [1 ]
Yang, Yang [1 ]
Yang, Nan [1 ]
Ma, Shuyun [1 ]
Xue, Fangfang [1 ]
Wang, Jianjun [4 ]
机构
[1] Xian Med Univ, Affiliated Hosp 1, Dept Obstet & Gynaecol, Xian 710077, Shaanxi, Peoples R China
[2] Shaanxi Univ Chinese Med, Affiliated Hosp, Dept Obstet & Gynaecol, Xianyang 712000, Shaanxi, Peoples R China
[3] Xian Med Univ, Affiliated Hosp 1, Dept Pharm, Xian 710077, Shaanxi, Peoples R China
[4] Xian Med Univ, Affiliated Hosp 1, Dept Emergency, 48 Fenghao West Rd, Xian 710077, Shaanxi, Peoples R China
关键词
Human neural stem cells; Oxygen and glucose deprivation; microRNA-21; Neuroprotection; STEM/PROGENITOR CELLS; ISCHEMIA; PROTECTS; PROLIFERATION; ACTIVATION; INJURY; BURDEN; MIR-21;
D O I
10.1016/j.neulet.2018.09.060
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Neural stem cells (NSCs) persist in the mammalian brain throughout life and protect against hypoxia-ischemia injury. NSCs are being increasingly recognized as a novel therapeutic target for various neurological disorders. Previous research indicates that miR-21 attenuates hypoxia-ischemia induced apoptotic death in various cell types. However, whether miR-21 plays a role in this protective effect mediated by NSCs is unknown, particularly in human NSCs (hNSCs). The present study investigated whether miR-21 could prevent hNSC injury induced by oxygen and glucose deprivation (OGD). Upon challenge with OGD treatment, loss of cell viability was observed in cultured hNSCs, as shown by CCK-8 assay. Moreover, quantitative real-time PCR (qRT-PCR) analysis indicated that expression of miR-21 increased in a time-dependent manner. TUNEL staining and Western blotting analysis showed that overexpression of miR-21 inhibited excessive hNSCs death induced by OGD treatment. Accordingly, knock down of miR-21 attenuated the neuroprotective effect observed in response to OGD treatment. Furthermore, JNK and p38 MAPKs inhibition was observed after overexpression of miR-21, and knock down of miR-21 had the opposite effect. We suggest that miR-21 prevents OGD-induced hNSCs death and apoptotic-associated protein activities through inhibiting JNK and p38 pathways in cultured hNSCs. Our findings may help to develop strategies for enhancing resident and transplanted NSCs survival after hypoxia-ischemic brain damage.
引用
收藏
页码:11 / 16
页数:6
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