Isolation method of Saccharomyces cerevisiae from red blood cells based on the optically induced dielectrophoresis technique for the rapid detection of fungal infections

被引:4
作者
Du, Mingao [1 ]
Liu, Fei [1 ]
Luan, Xiaoli [1 ]
Li, Gongxin [1 ,2 ]
机构
[1] Jiangnan Univ, Inst Automat, Minist Educ, Key Lab Adv Proc Control Light Ind, Wuxi 214122, Jiangsu, Peoples R China
[2] Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang 110016, Peoples R China
基金
中国国家自然科学基金;
关键词
FUNGEMIA; MICROPARTICLES; MANIPULATION; CAPACITANCE; PARTICLE; CTCS;
D O I
10.1364/BOE.448729
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Saccharomyces cerevisiae (S. cerevisiae) has been classically used to treat diarrhea and diarrhea-related diseases. However, in the past two decades, fungal infections caused by S. cerevisiae have been increasing among immunocompromised patients, and it takes too long to isolate S. cerevisiae from blood to diagnose it in time. In this paper, a new method for the isolation and selection of S. cerevisiae from red blood cells (RBC) is proposed by designing a microfluidic chip with an optically-induced dielectrophoresis (ODEP) system. It was verified by theory and experiments that the magnitude and direction of the dielectrophoresis force applied on RBCs and S. cerevisiae are different, which determine that the S. cerevisiae can be isolated from RBCs by the ODEP system. By designing the specific light images and the dynamic separation mode, the optimal operating conditions were experimentally achieved for acquiring higher purity of S. cerevisiae. The purity ranges were up to 95.9%-97.3%. This work demonstrates a promising tool for efficient and effective purification of S. cerevisiae from RBCs and provides a novel method of S. cerevisiae isolation for the timely diagnosis of fungal infections.
引用
收藏
页码:559 / 570
页数:12
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