Use of Pseudomonas fluorescens-based formulations for management of tomato spotted wilt virus (TSWV) and enhanced yield in tomato

被引：33

作者：

Kandan, A ^{[1
]}

Ramiah, M ^{[1
]}

Vasanthi, VJ ^{[1
]}

Radjacommare, R ^{[1
]}

Nandakumar, R ^{[1
]}

Ramanathan, A ^{[1
]}

Samiyappan, R ^{[1
]}

机构：

[1] Tamil Nadu Agr Univ, Ctr Plant Protect Studies, Dept Plant Pathol, Coimbatore 641003, Tamil Nadu, India

来源：

BIOCONTROL SCIENCE AND TECHNOLOGY | 2005年 / 15卷 / 06期

关键词：

biocontrol; induced systemic resistance; Pseudomonas fluorescens; tomato; TSWV;

D O I：

10.1080/09583150500088546

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Strains of Pseudomonas fluorescens were investigated for biocontrol efficacy against tomato spotted wilt virus ( TSWV) in tomato both alone and in mixtures. P. fluorescens strains applied to seed, soil and foliage or as a seedling dip significantly reduced TSWV, with a concomitant increase in growth promotion in both the glasshouse and field. Two native strains ( CoP-1 and CoT-1) and one foreign strain (CHAO) reduced TSWV. In P. fluorescens - treated tomato plants, increased activity of polyphenol oxidase, beta-1,3-glucanase and chitinase was observed, and induction of chitinase was confirmed by western blot analysis. Induction of new protein ( 18 kDa) detected by SDS - PAGE in P. fluorescens - treated tomato plants was not found in healthy and P. fluorescens - untreated virus inoculated control plants. Indirect ELISA clearly showed a reduction in viral antigen concentration in P. fluorescens - treated tomato plants corresponding to reduced disease ratings. All the P. fluorescens - treated tomato plants also showed enhanced growth and yield compared to control plants. Hence, plant growth promoting rhizobacteria ( PGPR) could play a major role in reducing TSWV and increasing yield in tomato plants.

引用

页码：553 / 569

页数：17

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