Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris

被引:10
作者
Fong, Mun Yik [1 ]
Lau, Yee Ling [2 ]
Zulqarnain, Mohamed [3 ]
机构
[1] Univ Malaya, Fac Med, Dept Parasitol, Kuala Lumpur 50603, Malaysia
[2] Monash Univ, Sch Arts & Sci, Bandar Sunway, Selangor, Malaysia
[3] Univ Malaya, Fac Med, Inst Biol Sci, Kuala Lumpur, Malaysia
关键词
Pichia pastoris; recombinant SAG2 antigen; Toxoplasma gondii;
D O I
10.1007/s10529-007-9609-x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The surface antigen 2 (SAG2) gene of the protozoan parasite, Toxoplasma gondii, was cloned and extracellularly expressed in the yeast Pichia pastoris. The effectiveness of the secreted recombinant SAG2 (rSAG2-S) as a serodiagnosis reagent was assessed by western blots and ELISA. In the western blot assay, rSAG2-S reacted with all Toxoplasma-antibody positive human serum samples but not with Toxoplasma-negative samples. In the ELISA, rSAG2-S yielded sensitivity rates ranging from 80% (IgG negative, IgM positive) to 100% (IgG positive, IgM negative). In vivo experiments showed that serum from mice immunized with rSAG2-S reacted specifically with the native SAG2 of T. gondii. These mice were protected when challenged with live cells of T. gondii.
引用
收藏
页码:611 / 618
页数:8
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