Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris

被引：10

作者：

Fong, Mun Yik ^{[1
]}

Lau, Yee Ling ^{[2
]}

Zulqarnain, Mohamed ^{[3
]}

机构：

[1] Univ Malaya, Fac Med, Dept Parasitol, Kuala Lumpur 50603, Malaysia

[2] Monash Univ, Sch Arts & Sci, Bandar Sunway, Selangor, Malaysia

[3] Univ Malaya, Fac Med, Inst Biol Sci, Kuala Lumpur, Malaysia

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 04期

关键词：

Pichia pastoris; recombinant SAG2 antigen; Toxoplasma gondii;

D O I：

10.1007/s10529-007-9609-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The surface antigen 2 (SAG2) gene of the protozoan parasite, Toxoplasma gondii, was cloned and extracellularly expressed in the yeast Pichia pastoris. The effectiveness of the secreted recombinant SAG2 (rSAG2-S) as a serodiagnosis reagent was assessed by western blots and ELISA. In the western blot assay, rSAG2-S reacted with all Toxoplasma-antibody positive human serum samples but not with Toxoplasma-negative samples. In the ELISA, rSAG2-S yielded sensitivity rates ranging from 80% (IgG negative, IgM positive) to 100% (IgG positive, IgM negative). In vivo experiments showed that serum from mice immunized with rSAG2-S reacted specifically with the native SAG2 of T. gondii. These mice were protected when challenged with live cells of T. gondii.

引用

页码：611 / 618

页数：8

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