Designer Biomaterials to Model Cancer Cell Invasion In Vitro: Predictive Tools or Just Pretty Pictures?

被引:9
作者
Bahlmann, Laura C. [1 ,2 ,3 ]
Smith, Laura J. [1 ,2 ,3 ]
Shoichet, Molly S. [1 ,2 ,3 ,4 ]
机构
[1] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5S 3G9, Canada
[2] Univ Toronto, Terrance Donnelly Ctr Cellular & Biomol Res, Toronto, ON M5S 3E1, Canada
[3] Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3E5, Canada
[4] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada
基金
加拿大健康研究院; 加拿大自然科学与工程研究理事会;
关键词
cancer cocultures; cell invasion; engineered hydrogels; extracellular matrix; tumor stromas; EXTRACELLULAR-MATRIX; TUMOR-MODEL; CARCINOMA-CELLS; STEM-CELL; MIGRATION; MICROENVIRONMENT; MACROPHAGES; HYDROGELS; FIBROBLASTS; HYALURONAN;
D O I
10.1002/adfm.201909032
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Metastasis is the leading cause of mortality in cancer patients. Underlying this process is the invasion and colonization of cancer cells into healthy tissues. Engineered hydrogel models of tumor microenvironments present an opportunity to understand the microenvironmental determinants of cellular invasion. The biochemical and mechanical cues, presented in the form of adhesion sites, degradable cues, matrix stiffness, and architecture, have significant effects on the extent of cancer cell migration, and the mechanisms employed by these cells to move through their matrix. Coculture with stromal cells such as cancer associated fibroblasts, endothelial cells, and immune cells that are associated with poor prognosis demonstrate that these cells exacerbate cancer cell invasion. With these models, researchers aim not only to recapitulate known cancer cell behaviors in a dish, but also to uncover new insights into mechanisms underlying these phenomena, paving the way for novel treatment strategies. In this perspective, the design of engineered models that are used to study cancer cell invasion and metastasis in vitro is discussed. To this end, the authors seek to understand and put into perspective: do these models reveal relevant mechanisms of cancer cell migration, or are they simply pretty pictures with little biological translatability?
引用
收藏
页数:11
相关论文
共 78 条
  • [1] Tumor macrophages are pivotal constructors of tumor collagenous matrix
    Afik, Ran
    Zigmond, Ehud
    Vugman, Milena
    Klepfish, Mordehay
    Shimshoni, Elee
    Pasmanik-Chor, Metsada
    Shenoy, Anjana
    Bassat, Elad
    Halpern, Zamir
    Geiger, Tamar
    Sagi, Irit
    Varol, Chen
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2016, 213 (11) : 2315 - 2331
  • [2] Elucidating the mechanobiology of malignant brain tumors using a brain matrix-mimetic hyaluronic acid hydrogel platform
    Ananthanarayanan, Badriprasad
    Kim, Yushan
    Kumar, Sanjay
    [J]. BIOMATERIALS, 2011, 32 (31) : 7913 - 7923
  • [3] Role of ErbB Receptors in Cancer Cell Migration and Invasion
    Appert-Collin, Aline
    Hubert, Pierre
    Cremel, Gerard
    Bennasroune, Amar
    [J]. FRONTIERS IN PHARMACOLOGY, 2015, 6
  • [4] Cancer-associated fibroblasts lead tumor invasion through integrin-β3-dependent fibronectin assembly
    Attieh, Youmna
    Clark, Andrew G.
    Grass, Carina
    Richon, Sophie
    Pocard, Marc
    Mariani, Pascale
    Elkhatib, Nadia
    Betz, Timo
    Gurchenkov, Basile
    Vignjevic, Danijela Matic
    [J]. JOURNAL OF CELL BIOLOGY, 2017, 216 (11) : 3509 - 3520
  • [5] 3D Traction Stresses Activate Protease-Dependent Invasion of Cancer Cells
    Aung, Aereas
    Seo, Young N.
    Lu, Shaoying
    Wang, Yingxiao
    Jamora, Colin
    del Alamo, Juan C.
    Varghese, Shyni
    [J]. BIOPHYSICAL JOURNAL, 2014, 107 (11) : 2528 - 2537
  • [6] Hyaluronan in peritumoral stroma and malignant cells associates with breast cancer spreading and predicts survival
    Auvinen, P
    Tammi, R
    Parkkinen, J
    Tammi, M
    Ågren, U
    Johansson, R
    Hirvikoski, P
    Eskelinen, M
    Kosma, VM
    [J]. AMERICAN JOURNAL OF PATHOLOGY, 2000, 156 (02) : 529 - 536
  • [7] Dynamic bioengineered hydrogels as scaffolds for advanced stem cell and organoid culture
    Bahlmann, Laura C.
    Fokina, Ana
    Shoichet, Molly S.
    [J]. MRS COMMUNICATIONS, 2017, 7 (03) : 472 - 486
  • [8] Benchmarking to the Gold Standard: Hyaluronan-Oxime Hydrogels Recapitulate Xenograft Models with In Vitro Breast Cancer Spheroid Culture
    Baker, Alexander E. G.
    Bahlmann, Laura C.
    Tam, Roger Y.
    Liu, Jeffrey C.
    Ganesh, Ahil N.
    Mitrousis, Nikolaos
    Marcellus, Richard
    Spears, Melanie
    Bartlett, John M. S.
    Cescon, David W.
    Bader, Gary D.
    Shoichet, Molly S.
    [J]. ADVANCED MATERIALS, 2019, 31 (36)
  • [9] Advantages of RGD peptides for directing cell association with biomaterials
    Bellis, Susan L.
    [J]. BIOMATERIALS, 2011, 32 (18) : 4205 - 4210
  • [10] Current mechanistic insights into the roles of matrix metalloproteinases in tumour invasion and metastasis
    Brown, Gordon T.
    Murray, Graeme I.
    [J]. JOURNAL OF PATHOLOGY, 2015, 237 (03) : 273 - 281