Refolding of denatured/reduced lysozyme using weak-cation exchange chromatography

被引：0
|
作者：
Wang, Y [1 ]
Gong, BL [1 ]
Geng, XD [1 ]
机构：
[1] NW Univ Xian, Key Lab Modern Separat Sci, Inst Modern Separat Sci, Xian 710069, Peoples R China
来源：
CHINESE CHEMICAL LETTERS | 2003年 / 14卷 / 08期
关键词：
weak-cation exchange chromatography; lysozyme; protein refolding;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Oxidative refolding of the denatured/reduced lysozyme was investigated by using weak-cation exchange chromatography (WCX). The stationary phase of WCX binds to the reduced lysozyme and prevented it from forming intermolecular aggregates. At the same time urea and ammonium sulfate were added to the mobile phase to increase the elution strength for lysozyme. Ammonium sulfate can more stabilize the native protein than a common eluting agent, sodium chloride. Refolding of lysozyme by using this WCX is successfully. It was simply carried out to obtain a completely and correctly refolding of the denatured lysozyme at high concentration of 20.0 mg/mL.
引用
收藏
页码：828 / 831
页数：4
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