Lipopolysaccharide-induced dental pulp cell apoptosis and the expression of Bax and Bcl-2 in vitro

被引:7
|
作者
Yang, H. [1 ]
Zhu, Y. T. [1 ,2 ]
Cheng, R. [1 ]
Shao, M. Y. [1 ]
Fu, Z. S. [1 ,3 ]
Cheng, L. [1 ]
Wang, F. M. [1 ]
Hu, T. [1 ]
机构
[1] Sichuan Univ, W China Coll Stomatol, State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China
[2] Guangzhou Med Univ, Stomatol Hosp, Guangzhou, Guangdong, Peoples R China
[3] Chongqing Med Univ, Affiliated Dent Hosp, Chongqing, Peoples R China
基金
中国国家自然科学基金;
关键词
Dental pulp cells; Lipopolysaccharide; Apoptosis; Bax; Bcl-2; ENDOTHELIAL-CELLS; NEUTROPHIL APOPTOSIS; TOOTH DEVELOPMENT; GROWTH-FACTOR; TNF-ALPHA; INHIBITION; FAMILY; REPAIR; DEATH; MACROPHAGES;
D O I
10.1590/S0100-879X2010007500102
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Lipopolysaccharide exerts many effects on many cell lines, including cytokine secretion, and cell apoptosis and necrosis. We investigated the in vitro effects of lipopolysaccharide on apoptosis of cultured human dental pulp cells and the expression of Bcl-2 and Bax. Dental pulp cells showed morphologies typical of apoptosis after exposure to lipopolysaccharide. Flow cytometry showed that the rate of apoptosis of human dental pulp cells increased with increasing lipopolysaccharide concentration. Compared with controls, lipopolysaccharide promoted pulp cell apoptosis (P < 0.05) from 0.1 to 100 mu g/mL but not at 0.01 mu g/ mL. Cell apoptosis was statistically higher after exposure to lipopolysaccharide for 3 days compared with 1 day, but no difference was observed between 3 and 5 days. Immunohistochemistry showed that expression of Bax and Bcl-2 was enhanced by lipopolysaccharide at high concentrations, but no evident expression was observed at low concentrations (0.01 and 0.1 mu g/mL) or in the control groups. In conclusion, lipopolysaccharide induced dental pulp cell apoptosis in a dose-dependent manner, but apoptosis did not increase with treatment duration. The expression of the apoptosis regulatory proteins Bax and Bcl-2 was also up-regulated in pulp cells after exposure to a high concentration of lipopolysaccharide.
引用
收藏
页码:1027 / 1033
页数:7
相关论文
共 50 条
  • [21] BCL-2/BAX expression in plasma cell dyscrasias.
    Renner, S
    Weisz, J
    Fleishman, A
    Taylor, J
    Vescio, R
    Rettig, M
    Berenson, J
    Krajewski, S
    Reed, JC
    Lichtenstein, A
    BLOOD, 1997, 90 (10) : 401 - 401
  • [22] Expression of p53, Bcl-2 and Bax in cisplatin-induced apoptosis in testicular germ cell tumour cell lines
    Burger, H
    Nooter, K
    Boersma, AWM
    Kortland, CJ
    Stoter, G
    BRITISH JOURNAL OF CANCER, 1998, 77 (10) : 1562 - 1567
  • [23] Expression of p53, Bcl-2 and Bax in cisplatin-induced apoptosis in testicular germ cell tumour cell lines
    H Burger
    K Nooter
    AWM Boersma
    CJ Kortland
    G Stoter
    British Journal of Cancer, 1998, 77 : 1562 - 1567
  • [24] Substance P enhances expression of lipopolysaccharide-induced inflammatory factors in dental pulp cells
    Tokuda, M
    Miyamoto, R
    Nagaoka, S
    Torii, M
    JOURNAL OF ENDODONTICS, 2004, 30 (11) : 770 - 774
  • [25] Growth factors prevent changes in Bcl-2 and Bax expression and neuronal apoptosis induced by nitric oxide
    Tamatani, M
    Ogawa, S
    Nuñez, G
    Tohyama, M
    CELL DEATH AND DIFFERENTIATION, 1998, 5 (10): : 911 - 919
  • [26] Apoptosis and expression of Bcl-2, Bax, and Caspase-3 in endometrial cancer
    Zhu, Xiaolu
    Wang, Juan
    Xu, Wei
    Xu, Yanli
    ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY, 2022, 18 : 27 - 27
  • [27] Quantitative expression of BCL-2 and BAX, as parameters of apoptosis in colorectal cancer tissue
    Veljkovic, A.
    Stanojevic, G.
    Branko, B.
    Sokolovic, D.
    Cvetanovic, A.
    Gordana, K.
    FEBS OPEN BIO, 2022, 12 : 190 - 190
  • [28] Growth factors prevent changes in Bcl-2 and Bax expression and neuronal apoptosis induced by nitric oxide
    Michio Tamatani
    Satoshi Ogawa
    Gabriel Nuñez
    Masaya Tohyama
    Cell Death & Differentiation, 1998, 5 : 911 - 919
  • [29] Bcl-2 but not Bax expression is associated with apoptosis in normal and transformed squamous epithelium
    Drenning, SD
    Marcovitch, AJ
    Johnson, DE
    Melhem, MF
    Tweardy, DJ
    Grandis, JR
    CLINICAL CANCER RESEARCH, 1998, 4 (11) : 2913 - 2921
  • [30] Apoptosis and expression of Bcl-2 and Bax proteins in invasive ductal carcinoma of the pancreas
    Nio, Y
    Iguchi, C
    Yamasawa, K
    Sasaki, S
    Takamura, M
    Toga, T
    Dong, M
    Itakura, M
    Tamura, K
    PANCREAS, 2001, 22 (03) : 230 - 239