Zika Virus Epidemic in Brazil. II. Post-Mortem Analyses of Neonates with Microcephaly, Stillbirths, and Miscarriage

被引：18

作者：

Azevedo, Raimunda S. S. ^{[1
]}

Araujo, Marialva T. ^{[1
]}

Oliveira, Consuelo S. ^{[1
,2
]}

Martins Filho, Arnaldo Jorge ^{[1
]}

Nunes, Bruno T. D. ^{[1
]}

Henriques, Daniele F. ^{[1
]}

Silva, Eliana V. P. ^{[1
]}

Carvalho, Valeria L. ^{[1
]}

Chiang, Jannifer O. ^{[1
]}

Martins, Livia C. ^{[1
]}

Vasconcelos, Barbara C. B. ^{[2
]}

Sousa, Jorge R. ^{[1
,3
]}

Araujo, Fernanda Montenegro C. ^{[4
]}

Ribeiro, Erlane M. ^{[5
]}

Castro, Andrezza R. P. ^{[5
]}

de Queiroz, Maria G. L. ^{[6
]}

Verotti, Mariana P. ^{[7
]}

Nunes, Marcio R. T. ^{[1
]}

Cruz, Ana C. R. ^{[1
]}

Rodrigues, Sueli G. ^{[1
]}

Shi, Pei-Yong ^{[8
]}

Quaresma, Juarez A. S. ^{[2
,3
]}

Tesh, Robert B. ^{[8
]}

Vasconcelos, Pedro F. C. ^{[1
,2
]}

机构：

[1] Minist Saude, Inst Evandro Chagas, Secao Arbovirol & Febres Hemorrag, Rodovia BR-316,Km-07, BR-67030000 Ananindeua, Brazil

[2] Univ Estado Para, Ctr Ciencias Biol & Saude, Travessa Perebebui 2623, BR-66087670 Belem, Para, Brazil

[3] Univ Fed Para, Programa Posgrad Doencas Trop, Ave Generalissimo Deodoro 92, BR-66055240 Belem, Para, Brazil

[4] Secretaria Saude Publ Ceara, Lab Cent, Ave Barao de Studart 2405, BR-60120002 Fortaleza, Ceara, Brazil

[5] Secretaria Saude Publ Ceara, Hosp Geral Cesar Cals, Ave Imperador 545, BR-60015152 Fortaleza, Ceara, Brazil

[6] Secretaria Saude Publ Rio Grande do Norte, Lab Cent, Rua Conego Monte 410, BR-59037170 Natal, RN, Brazil

[7] Fundacao Osvaldo Cruz Fiocruz, Fundacao Desenvolvimento Cient & Tecnol Saude FIO, SMPW Quadra 4 Conj 1 Lote 3 Unidade E, BR-71735401 Brasilia, DF, Brazil

[8] Univ Texas Med Branch, Dept Pathol, 301 Univ Blvd, Galveston, TX 77555 USA

来源：

JOURNAL OF CLINICAL MEDICINE | 2018年 / 7卷 / 12期

关键词：

Zika virus; microcephaly; congenital syndrome; ZIKV RNA; ZIKV antigen; HUMAN LIVER; INFECTION; DISEASE; DAMAGE;

D O I：

10.3390/jcm7120496

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Introduction: The recent Zika virus(ZIKV) epidemic in Brazil was characterized by a range of different clinical presentations, particularly microcephaly, Guillain-Barre syndrome, and death. In this context, we determined the causal relationship between fatal microcephaly cases and ZIKV infection. Methods: Twelve fatal cases of neonates, whose mothers were infected with ZIKV during pregnancy, were examined; cases included nine neonatal deaths due to microcephaly, one miscarriage, and two stillbirths. Tissue samples were obtained from all cases at necropsy and were submitted for virological investigation (RT-qPCR and virus isolation) and/or histopathology (hematoxylin and eosin staining) and immunohistochemical assay for the detection of ZIKV antigens. Results: ZIKV antigens and/or ZIKV RNA were detected in tissue samples of all 12 cases examined. ZIKV was recovered in one case. Results of the virological and immunohistochemical analyses, as well as the anatomic abnormalities and histopathologic changes observed at necropsy on the 12 fatal cases, are presented. Conclusions: Data from these 12 cases provide strong evidence of the causal relationship between ZIKV and congenital disease in fetuses of women who were infected with the virus during pregnancy.

引用

页数：13

共 32 条

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