Functional antagonism by a monoclonal antibody to digoxin in a test system of cultured rat heart myocytes

被引：2
|
作者：
Wallukat, G [1 ]
Simon, HU [1 ]
Muller, WD [1 ]
Wolf, I [1 ]
机构：
[1] UNIV JENA, INST CLIN IMMUNOL, D-07740 JENA, GERMANY
来源：
MOLECULAR AND CELLULAR BIOCHEMISTRY | 1996年 / 161卷
关键词：
glycosides; monoclonal antibodies; cardiac myocytes; cell culture; digitoxin intoxication;
D O I：
10.1007/BF00240040
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A monoclonal antibody with a high affinity for digitoxin (K-A = 0.50 nM) and digoxin (K-A = 0.55 nM) was produced by somatic cell fusion. This antibody, designated 2A3(47), displayed little cross reactivity with other glycosides. In cultured rat heart myocytes, 2A3(47), antagonized the positive chronotropic effect exerted by digitoxin but did not alter that of ouabain. Our results suggest that this monoclonal antibody may prove to be useful in treating digoxin and digitoxin intoxication.
引用
收藏
页码：117 / 120
页数：4
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