Fine Structure and Related Properties of the Assembleable Carbon Nanotubes Based Electrode for New Family of Biosensors with Chooseable Selectivity

被引:4
作者
Razumiene, Julija [1 ]
Gureviciene, Vidute [1 ]
Voitechovic, Edita [1 ]
Barkauskas, Jurgis [2 ]
Bukauskas, Virginijus [3 ]
Setkus, Arunas [3 ]
机构
[1] Inst Biochem, Dept Bioanal, LT-08662 Vilnius, Lithuania
[2] Vilnius Univ, Dept Gen & Inorgan Chem, LT-03225 Vilnius, Lithuania
[3] Inst Semicond Phys, Sensors Lab, LT-01108 Vilnius, Lithuania
关键词
SWCNT; Amperometric Biosensor; Enzymatic Membrane; Carbohydrates; Scanning Probe Microscopy; ELECTROCHEMICAL BIOSENSORS; GLUCOSE-DEHYDROGENASE;
D O I
10.1166/jnn.2011.3455
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Surfaces of constituent parts of biosensors based on single wall carbon nanotube layer were investigated and compare for properly functioning and faulty biosensors. Though the original technology is acceptable for changing of the selectivity, only glucose sensitive biosensors are investigated. Based on the results of the study, a correlation between the features of the nanoscale structures and parameters of amperiometric biosensors for assemblage of which an innovative approach is described. Original template of the electrodes has been prepared on a base of single wall carbon nanotube layer deposited or the supporting polycarbonate membrane. Original immobilisation of enzymes within special membrane allows functional modification of biosensors being accomplished by simple replacement of the enzymatic membrane. The original technology leads to a novel family of biosensors acceptable for detection of wide range of carbohydrates. The morphology and the local electric properties of the constituent parts of the biosensors are characterized by scanning probe microscopy. The sensitivity, selectivity and stability are described for typical types of the biosensors.
引用
收藏
页码:9003 / 9011
页数:9
相关论文
共 14 条
[1]   Mediated biosensors [J].
Chaubey, A ;
Malhotra, BD .
BIOSENSORS & BIOELECTRONICS, 2002, 17 (6-7) :441-456
[2]   Protein electrochemistry using aligned carbon nanotube arrays [J].
Gooding, JJ ;
Wibowo, R ;
Liu, JQ ;
Yang, WR ;
Losic, D ;
Orbons, S ;
Mearns, FJ ;
Shapter, JG ;
Hibbert, DB .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (30) :9006-9007
[3]   Binding of acetylcholinesterase to a multiwall carbon nanotube-cross-linked chitosan composite for flow-injection amperometric detection of an organophosphorous insecticide [J].
Kandimalla, VB ;
Ju, HX .
CHEMISTRY-A EUROPEAN JOURNAL, 2006, 12 (04) :1074-1080
[4]   Wiring of PQQ-dehydrogenases [J].
Laurinavicius, V ;
Razumiene, J ;
Ramanavicius, A ;
Ryabov, AD .
BIOSENSORS & BIOELECTRONICS, 2004, 20 (06) :1217-1222
[5]   Purification and characterisation of PQQ-dependent glucose dehydrogenase from Erwinia sp. 34-1 [J].
Marcinkeviciene, L ;
Bachmatova, I ;
Semenaite, R ;
Rudomanskis, R ;
Brazenas, G ;
Meskiene, R ;
Meskys, R .
BIOTECHNOLOGY LETTERS, 1999, 21 (03) :187-192
[6]   Quinoproteins: structure, functions and biotechnological applications [J].
Matsushita, K ;
Toyama, H ;
Yamada, M ;
Adachi, O .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2002, 58 (01) :13-22
[7]   Production, characterization, and reconstitution of recombinant quinoprotein glucose dehydrogenase (soluble type; EC 1.1.99.17) apoenzyme of Acinetobacter calcoaceticus [J].
Olsthoorn, AJJ ;
Duine, JA .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1996, 336 (01) :42-48
[8]   Direct electron transfer between PQQ dependent glucose dehydrogenases and carbon electrodes: An approach for electrochemical biosensors [J].
Razumiene, J. ;
Vilkanauskyte, A. ;
Gureviciene, V. ;
Barkauskas, J. ;
Meskys, R. ;
Laurinavicius, V. .
ELECTROCHIMICA ACTA, 2006, 51 (24) :5150-5156
[9]   Modified graphitized carbon black as transducing material for reagentless H2O2 and enzyme sensors [J].
Razumiene, J ;
Barkauskas, J ;
Kubilius, V ;
Meskys, R ;
Laurinavicius, V .
TALANTA, 2005, 67 (04) :783-790
[10]   Soluble aldose sugar dehydrogenase from Escherichia coli -: A highly exposed active site conferring broad substrate specificity [J].
Southall, Stacey M. ;
Doel, Justin J. ;
Richardson, David J. ;
Oubrie, Arthur .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (41) :30650-30659