Polypyrrole/phytase amperometric biosensors for the determination of phytic acid in standard solutions

被引：19

作者：

Rodrigues, Valquiria Cruz ^{[1
]}

de Moraes, Marli Leite ^{[2
]}

Brisolari, Andre ^{[1
]}

Soares, Juliana Coatrini ^{[1
]}

Ferreira, Marystela ^{[2
]}

Goncalves, Debora ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil

[2] Univ Fed Sao Carlos, BR-18052780 Sorocaba, SP, Brazil

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2011年 / 160卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

Biosensors; Phytase; Polypyrrole; Phytic acid detection; CONDUCTING POLYMERS; GLUCOSE-OXIDASE; PHYTASE; IMMOBILIZATION; PURIFICATION; SPECTROSCOPY; PHOSPHORUS; PROTECTION; PYRROLE; FILMS;

D O I：

10.1016/j.snb.2011.07.038

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Amperometric biosensors based on the physical immobilization of phytase (PhyA) into polypyrrole (PPy) films were prepared in aqueous medium. The PPy/PhyA films were characterized by cyclic voltammetry, and surface and structural characterization techniques, SEM and FTIR. Both voltammetric and amperometric transduction methods were used in order to detect phytic acid in acetate buffer at pH 5.5 at room temperature. The biosensors exhibited a detection limit of 0.15 mmol L(-1) and a linear range of phytic acid content from 0.5 to 2.0 mmol L(-1), which are adequate values for typical analyses of phytic acids in most seeds, grains, and vegetables. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：222 / 226

页数：5

共 29 条

[1] A novel staining method for detecting phytase activity [J].

Bae, HD ;

Yanke, LJ ;

Cheng, KJ ;

Selinger, LB .

JOURNAL OF MICROBIOLOGICAL METHODS, 1999, 39 (01) :17-22

[2] Fabrication of phytic acid sensor based on mixed phytase-lipid Langmuir-Blodgett films [J].

Caseli, Luciano ;

Moraes, Marli L. ;

Zucolotto, Valtencir ;

Ferreira, Marystela ;

Nobre, Thatyane M. ;

Zaniquelli, Maria Elisabete D. ;

Rodrigues Filho, Ubirajara P. ;

Oliveira, Osvaldo N., Jr. .

LANGMUIR, 2006, 22 (20) :8501-8508

[3]

Chivandi E, 2010, INT J AGRIC BIOL, V12, P785

[4] Purification and properties of extracellular phytase from Bacillus sp KHU-10 [J].

Choi, YM ;

Suh, HJ ;

Kim, JM .

JOURNAL OF PROTEIN CHEMISTRY, 2001, 20 (04) :287-292

[5] AN IR SPECTROSCOPIC STUDY OF THE ELECTROCHEMICAL REDUCTION OF POLYPYRROLE DOPED WITH DODECYL-SULFATE ANION [J].

DAVIDSON, RG ;

TURNER, TG .

SYNTHETIC METALS, 1995, 72 (02) :121-128

[6] Infrared and Raman spectroscopic studies of the electrochemical oxidative degradation of polypyrrole [J].

Ghosh, S ;

Bowmaker, GA ;

Cooney, RP ;

Seakins, JM .

SYNTHETIC METALS, 1998, 95 (01) :63-67

[7] Immobilization of invertase and glucose oxidase in conducting H-type polysiloxane/polypyrrole block copolymers [J].

Gürsel, A ;

Alkan, S ;

Toppare, L ;

Yagci, Y .

REACTIVE & FUNCTIONAL POLYMERS, 2003, 57 (01) :57-65

[8] Inositol phosphates from barley low-phytate grain mutants analysed by metal-dye detection HPLC and NMR [J].

Hatzack, F ;

Hübel, F ;

Zhang, W ;

Hansen, PE ;

Rasmussen, SK .

BIOCHEMICAL JOURNAL, 2001, 354 :473-480

[9]

Heindl U., 2006, AM SOYBEAN ASS, P19

[10] Biotechnological development of effective phytases for mineral nutrition and environmental protection [J].

X. Lei ;

C. Stahl .

Applied Microbiology and Biotechnology, 2001, 57 (4) :474-481

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