Cloning, Expression Analysis, 20-Hydroxyecdysone Induction, and RNA Interference Study of Autophagy-Related Gene 8 from Heortia vitessoides Moore
被引:11
作者:
Li, Zhixing
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South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R China
Li, Zhixing
[1
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Lyu, Zihao
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South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R China
Lyu, Zihao
[1
]
Ye, Qingya
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South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R China
Ye, Qingya
[1
]
Cheng, Jie
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South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R China
Cheng, Jie
[1
]
Wang, Chunyan
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South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R China
Wang, Chunyan
[1
]
Lin, Tong
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South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R China
Lin, Tong
[1
]
机构:
[1] South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Peoples R China
Autophagy is a highly conserved and regulated process in eukaryotic cells and remodels cytoplasm, recovers essential nutrients, and disposes of unwanted cytoplasmic components. Autophagy-related gene (ATG) 8, identified in Heortia vitessoides Moore, which is an oligophagous pest of Aquilaria sinensis (Lour.), was characterized (HvATG8). Multiple sequence alignment showed that HvATG8 possesses highly conserved domain structures. Stage- and tissue-specific expressions indicated that HvATG8 is highly expressed in prepupal, pupal, and adult stages and in the midgut of larvae and abdomen of adults. Lack of function of HvATG8 by RNA interference resulted in a significant decrease in survival rate and an increase in abnormal or nonviable phenotypes in H. vitessoides. Transition rate from larval to pupal stages was 33.0% and from pupal to adult stages was 15.0% after injection. Reduction of ATG8 expression reduced survival of H. vitessoides. Therefore, HvATG8 possibly plays a key role in normal growth stage of H. vitessoides. HvATG8 suppression downregulates HvATG3 expression, suggesting that the two genes are interconnected. Further, HvATG8 expression increased by 20-hydroxyecdysone treatment, starvation, and extreme temperature exposure. Starvation also altered expression of other ATGs in H. vitessoide. This study may be used to guide research on molecular mechanisms of autophagy in insects.
机构:
South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R ChinaSouth China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R China
Cheng, Jie
Chen, Jingxiang
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South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R ChinaSouth China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R China
Chen, Jingxiang
Lin, Tong
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South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R ChinaSouth China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R China
机构:
Albert Einstein Coll Med, Marion Bessin Liver Res Ctr, Dept Anat & Struct Biol, Bronx, NY 10461 USAAlbert Einstein Coll Med, Marion Bessin Liver Res Ctr, Dept Anat & Struct Biol, Bronx, NY 10461 USA
机构:
SA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, AustraliaSA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
Denton, Donna
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Shravage, Bhupendra
Simin, Rachel
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Univ Massachusetts, Sch Med, Dept Canc Biol, Worcester, MA 01605 USASA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
Simin, Rachel
Mills, Kathryn
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SA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, AustraliaSA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
Mills, Kathryn
Berry, Deborah L.
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机构:
Georgetown Univ, Lombardi Comprehens Canc Ctr, Washington, DC 20010 USASA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
Berry, Deborah L.
Baehrecke, Eric H.
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Univ Massachusetts, Sch Med, Dept Canc Biol, Worcester, MA 01605 USASA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
Baehrecke, Eric H.
Kumar, Sharad
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机构:
SA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
Univ Adelaide, Dept Med, Adelaide, SA 5005, AustraliaSA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
机构:
South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R ChinaSouth China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R China
Cheng, Jie
Chen, Jingxiang
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机构:
South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R ChinaSouth China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R China
Chen, Jingxiang
Lin, Tong
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机构:
South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R ChinaSouth China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R China
机构:
Albert Einstein Coll Med, Marion Bessin Liver Res Ctr, Dept Anat & Struct Biol, Bronx, NY 10461 USAAlbert Einstein Coll Med, Marion Bessin Liver Res Ctr, Dept Anat & Struct Biol, Bronx, NY 10461 USA
机构:
SA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, AustraliaSA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
Denton, Donna
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机构:
Shravage, Bhupendra
Simin, Rachel
论文数: 0引用数: 0
h-index: 0
机构:
Univ Massachusetts, Sch Med, Dept Canc Biol, Worcester, MA 01605 USASA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
Simin, Rachel
Mills, Kathryn
论文数: 0引用数: 0
h-index: 0
机构:
SA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, AustraliaSA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
Mills, Kathryn
Berry, Deborah L.
论文数: 0引用数: 0
h-index: 0
机构:
Georgetown Univ, Lombardi Comprehens Canc Ctr, Washington, DC 20010 USASA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
Berry, Deborah L.
Baehrecke, Eric H.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Massachusetts, Sch Med, Dept Canc Biol, Worcester, MA 01605 USASA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
Baehrecke, Eric H.
Kumar, Sharad
论文数: 0引用数: 0
h-index: 0
机构:
SA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia
Univ Adelaide, Dept Med, Adelaide, SA 5005, AustraliaSA Pathol, Dept Haematol, Ctr Canc Biol, Adelaide, SA 5000, Australia