Diagnosis of congenital toxoplasmosis by polymerase chain reaction on neonatal peripheral blood

被引:19
作者
Sterkers, Yvon [1 ]
Ribot, Jennifer
Albaba, Sahar
Issert, Eric
Bastien, Patrick
Pratlong, Francine
机构
[1] Univ Hosp Ctr Montpellier, Fac Med, Dept Parasitol Mycol, Montpellier, France
关键词
Toxoplasma gondii; PCR; Amniotic fluid; Placenta; Cord blood; MOLECULAR-DETECTION; GONDII INFECTION; AMNIOTIC-FLUID; AMPLIFICATION; PERFORMANCE;
D O I
10.1016/j.diagmicrobio.2011.06.006
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
In a cohort of 12 consecutive neonates, polymerase chain reaction (PCR) established the diagnosis of 5 of 6 cases of congenital toxoplasmosis and did so earlier than serologic methods. We validated that PCR using neonatal peripheral blood is a sensitive, rapid, and cost-effective method to affirm the diagnosis of previously undiagnosed congenital toxoplasmosis. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:174 / 176
页数:3
相关论文
共 13 条
  • [1] Three years of multi-laboratory external quality control for the molecular detection of Toxoplasma gondii in amniotic fluid in France
    Bastien, P.
    Jumas-Bilak, E.
    Varlet-Marie, E.
    Marty, P.
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2007, 13 (04) : 430 - 433
  • [2] Bastien Patrick, 2002, Trans R Soc Trop Med Hyg, V96 Suppl 1, pS205, DOI 10.1016/S0035-9203(02)90078-7
  • [3] Bergstrom T, 1998, SCAND J INFECT DIS, V30, P202, DOI 10.1080/003655498750003681
  • [4] DETECTION OF TOXOPLASMA-GONDII BY COMPETITIVE DNA AMPLIFICATION OF BRONCHOALVEOLAR LAVAGE SAMPLES
    BRETAGNE, S
    COSTA, JM
    VIDAUD, M
    VANNHIEU, JT
    FLEURYFEITH, J
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1993, 168 (06) : 1585 - 1588
  • [5] Comparison of two widely used PCR primer systems for detection of Toxoplasma in amniotic fluid, blood, and tissues
    Chabbert, E
    Lachaud, L
    Crobu, L
    Bastien, P
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (04) : 1719 - 1722
  • [6] DESMONTS G, 1974, B NEW YORK ACAD MED, V50, P146
  • [7] King L., 2008, Bulletin Epidemiologique Hebdomadaire, P122
  • [8] Classification system and case definitions of Toxoplasma gondii infection in immunocompetent pregnant women and their congenitally infected offspring
    Lebech, M
    Joynson, DHM
    Seitz, HM
    Thulliez, P
    Gilbert, RE
    Dutton, GN
    Ovlisen, B
    Petersen, E
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1996, 15 (10) : 799 - 805
  • [9] Antenatal diagnosis of congenital toxoplasmosis: Evaluation of the biological parameters in a cohort of 286 patients
    Pratlong, F
    Boulot, P
    Villena, I
    Issert, E
    Tamby, I
    Cazenave, J
    Dedet, JP
    [J]. BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 1996, 103 (06): : 552 - 557
  • [10] Performance of a Western blot assay to compare mother and newborn anti-toxoplasma antibodies for the early neonatal diagnosis of congenital toxoplasmosis
    Robert-Gangneux, F
    Commere, V
    Tourte-Schaefer, C
    Dupouy-Camet, J
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1999, 18 (09) : 648 - 654