Autologous NK cells as consolidation therapy following stem cell transplantation in multiple myeloma

被引：41

作者：

Nahi, Hareth ^{[1
,2
]}

Chrobok, Michael ^{[1
]}

Meinke, Stephan ^{[1
]}

Gran, Charlotte ^{[1
,3
]}

Marquardt, Nicole ^{[4
]}

Afram, Gabriel ^{[1
,2
]}

Sutlu, Tolga ^{[1
,14
]}

Gilljam, Mari ^{[1
]}

Stellan, Birgitta ^{[1
]}

Wagner, Arnika K. ^{[1
]}

Blomberg, Pontus ^{[5
,6
]}

Holmqvist, Per-Henrik ^{[6
]}

Walther-Jallow, Lilian ^{[7
]}

Mellstrom, Karin ^{[8
]}

Liwing, Johan ^{[8
]}

Gustafsson, Charlotte ^{[9
]}

Mansson, Robert ^{[9
]}

Klimkowska, Monika ^{[10
,11
]}

Gahrton, Gosta ^{[1
]}

Lund, Johan ^{[1
,2
]}

Ljungman, Per ^{[12
,13
]}

Ljunggren, Hans-Gustaf ^{[4
]}

Alici, Evren ^{[1
]}

机构：

[1] Karolinska Inst, Dept Med Huddinge, Ctr Hematol & Regenerat Med, SE-14183 Huddinge, Sweden

[2] Karolinska Univ Hosp, Dept Hematol, SE-14186 Huddinge, Sweden

[3] Karolinska Univ Lab, Dept Clin Chem, SE-14183 Huddinge, Sweden

[4] Karolinska Inst, Dept Med Huddinge, Ctr Infect Med, SE-14183 Huddinge, Sweden

[5] Karolinska Inst, Dept Lab Med, Vecura, SE-14186 Stockholm, Sweden

[6] Karolinska Univ Hosp, Karolinska Cell Therapy Ctr, Vecura, SE-14186 Stockholm, Sweden

[7] Karolinska Inst, Dept Clin Sci Intervent & Technol, SE-14183 Huddinge, Sweden

[8] XNK Therapeut AB, Halsovagen 7, SE-14157 Huddinge, Sweden

[9] Karolinska Inst, Div Clin Immunol & Transfus Med, Dept Lab Med, Ctr Hematol & Regenerat Med, SE-14183 Huddinge, Sweden

[10] Karolinska Inst, Dept Lab Med, Pathol Unit, SE-14183 Huddinge, Sweden

[11] Karolinska Univ Hosp, Dept Clin Pathol & Cytol, SE-14186 Huddinge, Sweden

[12] Karolinska Univ Hosp Huddinge, Dept Cellular Therapy & Allogene Stem Cell Transp, SE-14186 Huddinge, Sweden

[13] Karolinska Inst, Dept Med Huddinge, Div Hematol, SE-14183 Huddinge, Sweden

[14] Bogazici Univ, Dept Mol Biol & Genet, TR-34342 Bebek, Turkey

来源：

CELL REPORTS MEDICINE | 2022年 / 3卷 / 02期

基金：

瑞典研究理事会;

关键词：

NATURAL-KILLER-CELLS; H-2-DEFICIENT LYMPHOMA VARIANTS; INTERNATIONAL STAGING SYSTEM; ROOT GANGLIA NEURONS; MISSING SELF; ADOPTIVE TRANSFER; MEDIATED LYSIS; IMMUNOTHERAPY; EXPANSION; LEUKEMIA;

D O I：

10.1016/j.xcrm.2022.100508

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Few approaches have been made toward exploring autologous NK cells in settings of cancer immunotherapy. Here, we demonstrate the feasibility of infusing multiple doses of ex vivo activated and expanded autologous NK cells in patients with multiple myeloma (MM) post-autologous stem cell transplantation. Infused NK cells were detected in circulation up to 4 weeks after the last infusion. Elevations in plasma granzyme B levels were observed following each consecutive NK cell infusion. Moreover, increased granzyme B levels were detected in bone marrow 4 weeks after the last infusion. All measurable patients had objective, detectable responses after NK cell infusions in terms of reduction in M-component and/or minimal residual disease. The present study demonstrates that autologous NK cell-based immunotherapy is feasible in a setting of MM consolidation therapy. It opens up the possibility for usage of autologous NK cells in clinical settings where patients are not readily eligible for allogeneic NK cell-based immunotherapies.

引用

页数：16

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