Plasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range

被引：216

作者：

Tabakman, Scott M. ^{[1
]}

Lau, Lana ^{[1
]}

Robinson, Joshua T. ^{[1
]}

Price, Jordan ^{[2
]}

Sherlock, Sarah P. ^{[1
]}

Wang, Hailiang ^{[1
]}

Zhang, Bo ^{[1
]}

Chen, Zhuo ^{[3
]}

Tangsombatvisit, Stephanie ^{[2
]}

Jarrell, Justin A. ^{[2
]}

Utz, Paul J. ^{[2
]}

Dai, Hongjie ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Dept Med, Div Rheumatol & Immunol, Stanford, CA 94305 USA

[3] Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

来源：

NATURE COMMUNICATIONS | 2011年 / 2卷

基金：

美国国家卫生研究院;

关键词：

METAL-ENHANCED FLUORESCENCE; ANTIBODIES; CELLS; TOOL;

D O I：

10.1038/ncomms1477

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Protein chips are widely used for high-throughput proteomic analysis, but to date, the low sensitivity and narrow dynamic range have limited their capabilities in diagnostics and proteomics. Here we present protein microarrays on a novel nanostructured, plasmonic gold film with near-infrared fluorescence enhancement of up to 100-fold, extending the dynamic range of protein detection by three orders of magnitude towards the fM regime. We employ plasmonic protein microarrays for the early detection of a cancer biomarker, carcinoembryonic antigen, in the sera of mice bearing a xenograft tumour model. Further, we demonstrate a multiplexed autoantigen array for human autoantibodies implicated in a range of autoimmune diseases with superior signal-to-noise ratios and broader dynamic range compared with commercial nitrocellulose and glass substrates. The high sensitivity, broad dynamic range and easy adaptability of plasmonic protein chips presents new opportunities in proteomic research and diagnostics applications.

引用

页数：9

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