Design and activity study of a melittin-thanatin hybrid peptide

被引：16

作者：

Jiang, Xiaofeng ^{[1
,4
,5
]}

Qian, Kun ^{[2
]}

Liu, Guangping ^{[1
,3
]}

Sun, Laiyu ^{[2
]}

Zhou, Guoqing ^{[2
]}

Li, Jingfen ^{[2
]}

Fang, Xinqiang ^{[2
]}

Ge, Haixia ^{[2
]}

Lv, Zhengbing ^{[1
,5
]}

机构：

[1] Zhejiang Sci Tech Univ, Coll Life Sci & Med, Hangzhou 310018, Zhejiang, Peoples R China

[2] Huzhou Univ, Sch Life Sci, Huzhou 313000, Zhejiang, Peoples R China

[3] Yanan Univ, Coll Life Sci, Yanan 716000, Shaanxi, Peoples R China

[4] Zhejiang Univ, Sch Life Sci, Hangzhou 31058, Zhejiang, Peoples R China

[5] Hangzhou Huadihlth Grp Ltd Co, Hangzhou 31012, Zhejiang, Peoples R China

来源：

AMB EXPRESS | 2019年 / 9卷 / 1期

关键词：

Melittin; Thanatin; Hybrid peptide; Structure; Activity; INDUCED CYTOGENETIC DAMAGE; GRAM-NEGATIVE BACTERIA; ANTIMICROBIAL PEPTIDE; IN-VITRO; ANTIBACTERIAL; ANALOGS; EXPRESSION; FUSION; CYTOTOXICITY; PROTEGRIN-1;

D O I：

10.1186/s13568-019-0739-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The unique antimicrobial mechanism of antimicrobials make them a promising substitute for antibiotics for fighting drug-resistant bacteria. Both melittin and thanatin have antimicrobial bioactivity. However, thanatin does not inhibit the growth of Staphylococcus aureus. Melittin can inhibit S. aureus and has strong hemolytic activity. In the present study, the mutant fragments of melittin and thanatin were combined by flexible peptides to form a novel hybrid peptide, which was synthesized based on the secondary and tertiary structure prediction. The hybrid peptide inhibited S. aureus with a hemolytic concentration of above 45mol/L and inhibition rate in SMMC-7721 cells of 19.14%. The hybrid antimicrobial peptide, which was designed by the combination of -helix and -lamellar antimicrobial peptides, showed that both types of peptides did not interact with each either on spatial structure or biological activities, thereby providing a novel idea for the design of artificial antimicrobial peptides.

引用

页数：6

共 39 条

[1] A new hybrid bacteriocin, Ent35-MccV, displays antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria [J].

Acuna, Leonardo ;

Picariello, Gianluca ;

Sesma, Fernando ;

Morero, Roberto D. ;

Bellomio, Augusto .

FEBS OPEN BIO, 2012, 2 :12-19

[2] Dissection of antibacterial and toxic activity of melittin - A leucine zipper motif plays a crucial role in determining its hemolytic activity but not antibacterial activity [J].

Asthana, N ;

Yadav, SP ;

Ghosh, JK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (53) :55042-55050

[3] HEMOLYTIC AND ANTIMICROBIAL ACTIVITIES OF THE 24 INDIVIDUAL OMISSION ANALOGS OF MELITTIN [J].

BLONDELLE, SE ;

HOUGHTEN, RA .

BIOCHEMISTRY, 1991, 30 (19) :4671-4678

[4] Multiscale Models of the Antimicrobial Peptide Protegrin-1 on Gram-Negative Bacteria Membranes [J].

Bolintineanu, Dan S. ;

Vivcharuk, Victor ;

Kaznessis, Yiannis N. .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2012, 13 (09) :11000-11011

[5] The Novel Biological Action of Antimicrobial Peptides via Apoptosis Induction [J].

Cho, Jaeyong ;

Hwang, In-sok ;

Choi, Hyemin ;

Hwang, Ji Hong ;

Hwang, Jae-Sam ;

Lee, Dong Gun .

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 22 (11) :1457-1466

[6] Synthesis and Antitubercular Activity of Novel Amino Acid Derivatives [J].

Da Costa, Cristiane F. ;

Pinheiro, Alessandra C. ;

De Almeida, Mauro V. ;

Lourenco, Maria C. S. ;

De Souza, Marcus V. N. .

CHEMICAL BIOLOGY & DRUG DESIGN, 2012, 79 (02) :216-222

[7] Machine-Learning Techniques Applied to Antibacterial Drug Discovery [J].

Durrant, Jacob D. ;

Amaro, Rommie E. .

CHEMICAL BIOLOGY & DRUG DESIGN, 2015, 85 (01) :14-21

[8] The antibiotic Furvina® targets the P-site of 30S ribosomal subunits and inhibits translation initiation displaying start codon bias [J].

Fabbretti, Attilio ;

Brandi, Letizia ;

Petrelli, Dezemona ;

Pon, Cynthia L. ;

Castanedo, Nilo R. ;

Medina, Ricardo ;

Gualerzi, Claudio O. .

NUCLEIC ACIDS RESEARCH, 2012, 40 (20) :10366-10374

[9] Structure-activity analysis of thanatin, a 21-residue inducible insect defense peptide with sequence homology to frog skin antimicrobial peptides [J].

Fehlbaum, P ;

Bulet, P ;

Chernysh, S ;

Briand, JP ;

Roussel, JP ;

Letellier, L ;

Hetru, C ;

Hoffmann, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (03) :1221-1225

[10] Melittin induced cytogenetic damage, oxidative stress and changes in gene expression in human peripheral blood lymphocytes [J].

Gajski, Goran ;

Domijan, Ana-Marija ;

Zegura, Bojana ;

Stern, Alja ;

Geric, Marko ;

Jovanovic, Ivana Novak ;

Vrhovac, Ivana ;

Madunic, Josip ;

Breljak, Davorka ;

Filipic, Metka ;

Garaj-Vrhovac, Vera .

TOXICON, 2016, 110 :56-67

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