Analysis of Peptide Mixtures for Proteomics Research Using LC-ESI-MS with a Simple Microgradient Device

被引：0

作者：

Lenobel, Rene ^{[1
]}

Rehulkova, Helena ^{[2
]}

Sebela, Marek ^{[1
]}

Franc, Vojtech ^{[1
]}

Kahle, Vladislav ^{[3
]}

Moravcova, Dana ^{[3
]}

Rehulka, Pavel ^{[2
]}

机构：

[1] Palacky Univ, Fac Sci, Ctr Reg Hana Biotechnol & Agr Res, Dept Prot Biochem & Prote, CR-77147 Olomouc, Czech Republic

[2] Univ Def, Fac Mil Hlth Sci, Dept Mol Pathol & Biol, Hradec Kralove, Czech Republic

[3] ASCR, Vvi, Inst Analyt Chem, Brno, Czech Republic

来源：

LC GC NORTH AMERICA | 2015年 / 33卷 / 06期

关键词：

LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; FRANCISELLA-TULARENSIS; PROTEINS; GLYCOSYLATION; IDENTIFICATION; PRINCIPLES; SEPARATION; OFFLINE; MS/MS;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This work presents an on-line combination of a simple microgradient device for reversed-phase liquid chromatography (LC) and electrospray ionization (ESI) tandem mass spectrometry (MS-MS). The formation of an S-shaped gradient of the strong mobile phase (based on acetonitrile) inside a microsyringe is described under the conditions applied for capillary separation of proteomic samples and their analysis using ESI-MS-MS on a hybrid instrument. The system was successfully tested for a complex peptide mixture originating from the digestion of the whole-cell lysate from Francisella tularensis subsp. Schu 54 and it represents a cheap alternative for the separation of both simple and complex peptide mixtures before MS analysis.

引用

页码：420 / +

页数：7

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