Anti-inflammatory effect of Chunkoongkeigi-tang on IL-1-induced inflamed A549 by the inhibition of COX-2 expression

被引:4
作者
An, Eun-Jin [1 ,2 ]
Kim, Kwanil [3 ]
Kwon, Daeho [4 ]
Jang, Hyeung-Jin [1 ,2 ]
机构
[1] Kyung Hee Univ, Coll Korean Med, 26 Kyungheedae Ro, Seoul 02447, South Korea
[2] Kyung Hee Univ, Grad Sch, Dept Sci Korean Med, 26 Kyungheedae Ro, Seoul 02447, South Korea
[3] Kyung Hee Univ, Coll Korean Med, Dept Internal Med, Div Allergy Immune & Resp Syst, 26 Kyungheedae Ro, Seoul 02447, South Korea
[4] Catholic Kwandong Univ, Dept Microbiol, Coll Med, Kangnung 25601, South Korea
基金
新加坡国家研究基金会;
关键词
Chunkoong-keigitang (CKT); Inflammatory lung diesases; COX-2; MAPK; NF-B; Pro-inflammatory cytokines; Asthma; MOLECULAR-MECHANISMS; AIRWAY INFLAMMATION; P38; MAPK; CELLS; PAEONIFLORIN; INVOLVEMENT; PATHWAYS; ASTHMA; MICE; INOS;
D O I
10.1007/s13273-019-0011-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
BackgroundsInflammatory lung disease can arise due to the immoderate expression of pro-inflammatory genes and the disharmony of complex cytokines. Chunkoongkeigi-tang (CKT) has been prescribed to patients with asthmatic symptoms in herbal medicine clinics. However, the effects of CKT on the inflamed human lung cell line A549 have yet to be revealed. Thus we investigate whether CKT can suppress inflammatory response on interleukin 1 (IL-1)-induced inflamed A549.MethodsIn an effort to understand the inhibitory activity of CKT on inflamed A549, we use IL-1 to induce inflammatory gene COX-2 expressions on A549. Inhibition of COX-2 expression and inflammatory cytokine secretion through blocking mitogen activated protein kinase (MAPK) pathway and transcriptional nuclear factor kappa B (NF-B) activity was confimed by western blot and real-time PCR. Compounds in CKT were identified by liquid chromatography-mass spectrometry (LC-MS).ResultsCKT inhibited increased COX-2 in an IL-1-induced inflammatory state of A549. We confirmed that CKT regulated the mitogen activated protein kinase (MAPK) pathway and transcriptional nuclear factor kappa B (NF-B). Additionally, a suppressive effect of CKT on the mRNA expression levels of cytokine-related asthma ((IL-5, IL-6, IL-17 and TNF-) was noted in IL-1-induced inflamed A549. According to LC-MS analysis, we identified several components in CKT which can be expected to be the active compounds of the anti-inflammatory effect of CKT.ConclusionWe verify the anti-inflammatory effect of CKT via the reduction of the IL-1-induced increased COX-2 expression through the MAPK and NF-B pathways, suggesting useful anti-asthmatic potential by inhibiting inflammatory cytokines, especially in patients with asthma.
引用
收藏
页码:93 / 102
页数:10
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