Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocyst

被引：82

作者：

Gerbe, Francois ^{[1
]}

Cox, Brian ^{[2
,3
]}

Rossant, Janet ^{[2
,3
]}

Chazaud, Claire ^{[1
]}

机构：

[1] INSERM, UMR384, Fac Med, F-63001 Clermont Ferrand, France

[2] Hosp Sick Children, Dept Dev & Stem Cell Biol, Toronto, ON M5G 1X8, Canada

[3] Univ Toronto, Dept Mol & Med Genet, Toronto, ON, Canada

来源：

DEVELOPMENTAL BIOLOGY | 2008年 / 313卷 / 02期

基金：

加拿大健康研究院;

关键词：

blastocyst; epithelium; cell differentiation; primitive endoderm; cell polarization; microarray;

D O I：

10.1016/j.ydbio.2007.10.048

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mesenchyme to epithelium transitions are crucial to embryonic development. The early mouse embryo offers an excellent model to study epithelium formation as during the first three days of development two epithelia are formed, the trophectoderm (TE) and the primitive endoderm (PrE). We have previously shown that PrE cells are determined within the blastocyst ICM long before epithelium formation. In this work, we isolated Lrp2 as a novel PrE precursor (pre-PrE) marker by using a microarray strategy that combines a transcriptome analysis of three stem cell lines and early embryos. A detailed expression analysis shows that Lrp2 expression is induced in late E3.5 embryos indicating that pre-PrE cells are progressively maturing prior to polarization into an epithelium. Furthermore, the subcellular location of Lrp2, Disabled-2 (Dab2) and Collagen-IV shows that the epithelial structure is acquired in individual cells through successive steps. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：594 / 602

页数：9