Detection of Schistosoma mansoni membrane antigens by immunoblot analysis of sera of patients from low-transmission areas

被引:18
作者
Cesari, IM [1 ]
Ballen, DE [1 ]
Mendoza, L [1 ]
Matos, C [1 ]
机构
[1] Inst Venezolano Invest Cient, Lab Immunoparasitol, Ctr Microbiol & Biol Celular, Caracas 1020A, Venezuela
关键词
D O I
10.1128/CDLI.12.2.280-286.2005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Schistosoma mansoni surface membrane components play a relevant role in the host-parasite interaction, and some are released in vivo as circulating antigens. n-Butanol extraction favors the release of membrane antigens like alkaline phosphatase, which has been shown to be specifically recognized by antibodies from S. mansoni-infected humans and animals. In the present study, components in the n-butanol extract (BE) of the adult S. mansoni worm membrane fraction were separated by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D SDS-PAGE [15%]) and further analyzed by immunoblotting (immunoglobulin G) using defined sera. S. mansoni-infected patient sera, but not sera of uninfected patients or sera obtained from patients infected with other parasite species, specifically and variably recognized up to 20 polypeptides in the molecular mass range of similar to 8 to > 80 kDa. There were some differences in the number, intensity, and frequency of recognition of the BE antigens among sera from Venezuelan sites of endemicity with a different status of schistosomiasis transmission. Antigens in the 28- to 24-kDa molecular mass range appeared as immunodominants and were recognized by S. mansoni-positive sera from all the sites, with recognition frequencies varying between 57.5 and 97.5%. Immunoblotting with BE membrane antigens resulted in a highly sensitive (98.1%), specific (96.1.0%), and confirmatory test for the immunodiagnosis of schistosomiasis in low-transmission areas.
引用
收藏
页码:280 / 286
页数:7
相关论文
共 27 条
[1]   Partial molecular characterization of Sm8, a tegumental antigen of Schistosoma mansoni [J].
Abath, FGC ;
Xavier, EM ;
Montenegro, SML ;
Werkhauser, RP .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2002, 97 :91-93
[2]   Characterization of Sm13, a tegumental antigen of Schistosoma mansoni [J].
Abath, FGC ;
Xavier, EM ;
Allen, R ;
Gomes, YM ;
Lucena-Silva, N ;
Baliza, M ;
Simpson, AJG .
PARASITOLOGY RESEARCH, 2000, 86 (09) :745-752
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   IMMUNITY AFTER TREATMENT OF HUMAN SCHISTOSOMIASIS MANSONI .2. IDENTIFICATION OF RESISTANT INDIVIDUALS, AND ANALYSIS OF THEIR IMMUNE-RESPONSES [J].
BUTTERWORTH, AE ;
CAPRON, M ;
CORDINGLEY, JS ;
DALTON, PR ;
DUNNE, DW ;
KARIUKI, HC ;
KIMANI, G ;
KOECH, D ;
MUGAMBI, M ;
OUMA, JH ;
PRENTICE, MA ;
RICHARDSON, BA ;
SIONGOK, TKA ;
STURROCK, RF ;
TAYLOR, DW .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1985, 79 (03) :393-408
[5]  
CESARI I M, 1987, Memorias do Instituto Oswaldo Cruz, V82, P175, DOI 10.1590/S0074-02761987000800030
[6]  
Cesari I. M., 2002, Boletin de Malariologia y Saneamiento Ambiental, V42, P29
[7]  
CESARI IM, 1992, DIAGNOSTIC APPROACHE, P83
[8]   The last fifteen years of schistosomiasis in Venezuela: Features and evolution [J].
de Noya, BA ;
Balzan, C ;
Arteaga, C ;
Cesari, I ;
Noya, O .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1999, 94 (02) :139-146
[9]   Evaluation of alkaline phosphatase immunoassay and comparison with other diagnostic methods in areas of low transmission of schistosomiasis [J].
deNoya, BA ;
Cesari, IM ;
Losada, S ;
Colmenares, C ;
Balzan, C ;
Hoebeke, J ;
Noya, O .
ACTA TROPICA, 1997, 66 (02) :69-78
[10]  
DENOYA BA, 2003, BOL MALARIOL SAN AMB, V43, P21