Modeling of expanded-bed protein adsorption by taking into account the axial particle size distribution

A mathematical model was established to describe protein adsorption behavior in expanded-bed chromatography by taking into account the axial size classification of adsorbent within the bed. This model has been used to simulate the expanded-bed adsorption (EBA) of bovine serum albumin (BSA) to Streamline DEAE under various operating conditions, such as different feed protein concentrations, liquid velocities and liquid viscosities. The parameters involved in the model are determined by the independent experiments or calculated from correlation to ensure a reliable comparison between the experimental and simulated results. Using these independently determined parameters, the model prediction agreed reasonably well to the experimental data. Moreover, the model was found more precise than that without considering the axial particle size distribution. (C) 2003 Elsevier Science B.V. All rights reserved.