Fibroblast-led collective invasion of carcinoma cells with differing roles for RhoGTPases in leading and following cells

被引：1185

作者：

Gaggioli, Cedric

Hooper, Steven

Hidalgo-Carcedo, Cristina

Grosse, Robert

Marshall, John F.

Harrington, Kevin

Sahai, Erik

机构：

[1] Canc Res UK London Res Inst, London WC2A 3PX, England

[2] Heidelberg Univ, Inst Pharmacol, D-69120 Heidelberg, Germany

[3] Barts & London Med & Dent Sch, Queen Marys Coll, Canc Res UK Clin Ctr, Tumour Biol Ctr, London EC1M 6BQ, England

[4] Inst Canc Res, Canc Res UK Ctr Cell & Mol Biol, Chester Beatty Labs, London SW3 6JB, England

来源：

NATURE CELL BIOLOGY | 2007年 / 9卷 / 12期

关键词：

D O I：

10.1038/ncb1658

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Imaging of collectively invading cocultures of carcinoma cells and stromal fibroblasts reveals that the leading cell is always a fibroblast and that carcinoma cells move within tracks in the extracellular matrix behind the fibroblast. The generation of these tracks by fibroblasts is sufficient to enable the collective invasion of the squamous cell carcinoma ( SCC) cells and requires both protease- and force-mediated matrix remodelling. Force-mediated matrix remodelling depends on integrins alpha 3 and alpha 5, and Rho-mediated regulation of myosin light chain ( MLC) activity in fibroblasts, but these factors are not required in carcinoma cells. Instead, carcinoma cells use Cdc42 and MRCK ( myotonic dystrophy kinase-related CDC42-binding protein kinases) mediated regulation of MLC to follow the tracks generated by fibroblasts.

引用

页码：1392 / U92

页数：19

共 33 条

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