Retinyl acetate-loaded nanoparticles: Dermal penetration and release of the retinyl acetate

被引:26
作者
Arayachukeat, Sunatda [2 ,3 ]
Wanichwecharungruang, Supason P. [1 ]
Tree-Udom, Thapakorn [3 ]
机构
[1] Chulalongkorn Univ, Fac Sci, Dept Chem, Bangkok 10330, Thailand
[2] Chulalongkorn Univ, Natl Ctr Petr Petrochem & Adv Mat, Bangkok 10330, Thailand
[3] Chulalongkorn Univ, Program Petrochem & Polymer Sci, Bangkok 10330, Thailand
关键词
Retinyl acetate; Encapsulation; Hair follicle; Polymeric carrier; Dermal penetration; Drug release; SOLID LIPID NANOPARTICLES; ALL-TRANS RETINOL; STRATUM-CORNEUM; TRETINOIN; SKIN; PALMITATE; DELIVERY; ACID; PHOTODECOMPOSITION; MICROEMULSION;
D O I
10.1016/j.ijpharm.2010.11.019
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Retinyl acetate (RA) loaded polymeric nanoparticle (NP) carriers were prepared using two different single polymers, ethyl cellulose (EC) and poly (ethylene glycol)-4-methoxycinnamoylphthaloylchitosan (PCPLC). The stability of RA to aqueous solution and UVA light was significantly improved when encapsulated with PCPLC, whilst EC encapsulation gave some improved stability in water but showed no improved photostability. Ex vivo application of free RA and the RA-loaded PCPLC NPs onto the surface of the freshly excised skin from a baby mouse indicated a significantly slower skin absorption rate for the encapsulated RA. However, 100% retention of the encapsulated RA in the skin tissue was observed after 24 h. Confocal fluorescent analysis of the skin pieces applied with the RA-loaded PCPLC NPs indicated likely entry and accumulation of the PCPLC NPs and RA at the hair follicles. Release of RA from the PCPLC NP carriers was confirmed through the detection of an increasingly higher RA/PCPLC fluorescent signal ratio deeper into the dermis and away from the hair follicles. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:281 / 288
页数:8
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