Photographed Rapid HIV Test Results Pilot Novel Quality Assessment and Training Schemes

被引:13
作者
Chiu, Yu-Ho C. [1 ]
Ong, Joanna [1 ]
Walker, Sandy [1 ]
Kumalawati, July [2 ]
Gartinah, Tintin [3 ]
McPhee, Dale A. [1 ,4 ]
Dax, Elizabeth M. [1 ,4 ]
机构
[1] St Vincents Inst, NRL, Melbourne, Vic, Australia
[2] Univ Indonesia, Dept Clin Pathol, Fac Med, Dr Cipto Mangunkusumo Hosp, Jakarta, Indonesia
[3] W Java Hlth Off, W Java Prov Hlth Lab, Bandung, Indonesia
[4] Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3052, Australia
来源
PLOS ONE | 2011年 / 6卷 / 03期
关键词
ASSAYS; SETTINGS;
D O I
10.1371/journal.pone.0018294
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
HIV rapid diagnostic tests (RDTs) are now used widely in non-laboratory settings by non-laboratory-trained operators. Quality assurance programmes are essential in ensuring the quality of HIV RDT outcomes. However, there is no cost-effective means of supplying the many operators of RDTs with suitable quality assurance schemes. Therefore, it was examined whether photograph-based RDT results could be used and correctly interpreted in the non-laboratory setting. Further it was investigated if a single training session improved the interpretation skills of RDT operators. The photographs were interpreted, a 10-minute tutorial given and then a second interpretation session was held. It was established that the results could be read with accuracy. The participants (n = 75) with a range of skills interpreted results (> 80% concordance with reference results) from a panel of 10 samples (three negative and seven positive) using four RDTs. Differences in accuracy of interpretation before and after the tutorial were marked in some cases. Training was more effective for improving the accurate interpretation of more complex results, e. g. results with faint test lines or for multiple test lines, and especially for improving interpretation skills of inexperienced participants. It was demonstrated that interpretation of RDTs was improved using photographed results allied to a 10-minute training session. It is anticipated that this method could be used for training but also for quality assessment of RDT operators without access to conventional quality assurance or training schemes requiring wet samples.
引用
收藏
页数:7
相关论文
共 15 条
  • [1] Inaccurate Diagnosis of HIV-1 Group M and O Is a Key Challenge for Ongoing Universal Access to Antiretroviral Treatment and HIV Prevention in Cameroon
    Aghokeng, Avelin F.
    Mpoudi-Ngole, Eitel
    Dimodi, Henriette
    Atem-Tambe, Arrah
    Tongo, Marcel
    Butel, Christelle
    Delaporte, Eric
    Peeters, Martine
    [J]. PLOS ONE, 2009, 4 (11):
  • [2] [Anonymous], GUID APPR EV HIV TES
  • [3] Implementing Rapid HIV Testing in Outreach and Community Settings: Results from an Advancing HIV Prevention Demonstration Project Conducted in Seven US Cities
    Bowles, Kristina E.
    Clark, Hollie A.
    Tai, Eric
    Sullivan, Patrick S.
    Song, Binwei
    Tsang, Jenny
    Dietz, Craig A.
    Mir, Julita
    Mares-DelGrasso, Azul
    Calhoun, Cindy
    Aguirre, Daisy
    Emerson, Cicily
    Heffelfinger, James D.
    [J]. PUBLIC HEALTH REPORTS, 2008, 123 : 78 - 85
  • [4] Immunoassays for the diagnosis of HIV: meeting future needs by enhancing the quality of testing
    Chappel, Roderick J.
    Wilson, Kim M.
    Dax, Elizabeth M.
    [J]. FUTURE MICROBIOLOGY, 2009, 4 (08) : 963 - 982
  • [5] Suitability of Simple Human Immunodeficiency Virus Rapid Tests in Clinical Trials in Community-Based Clinic Settings
    Everett, Dean B.
    Baisley, Kathy
    Changalucha, John
    Vallely, Andrew
    Watson-Jones, Deborah
    Cook, Claire
    Knight, Louise
    Ross, David A.
    Mugeye, Kokugonza
    McCormack, Sheena
    Lacey, Charles J.
    Jentsch, Ute
    Hayes, Richard J.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (04) : 1058 - 1062
  • [6] Evaluation of rapid tests for anti-HIV detection in Brazil
    Junior, OCF
    Ferreira, C
    Riedel, M
    Widolin, MRV
    Barbosa-Júnior, A
    [J]. AIDS, 2005, 19 : S70 - S75
  • [7] Rapid vs. Standard HIV Testing in Bathhouses: What is Gained and Lost?
    Huebner, David M.
    Binson, Diane
    Dilworth, Samantha E.
    Neilands, Torsten B.
    Grinstead, Olga
    Woods, William J.
    [J]. AIDS AND BEHAVIOR, 2010, 14 (03) : 688 - 696
  • [8] The Evaluation of a Rapid In Situ HIV Confirmation Test in a Programme with a High Failure Rate of the WHO HIV Two-Test Diagnostic Algorithm
    Klarkowski, Derryck B.
    Wazome, Joseph M.
    Lokuge, Kamalini M.
    Shanks, Leslie
    Mills, Clair F.
    O'Brien, Daniel P.
    [J]. PLOS ONE, 2009, 4 (02):
  • [9] Assessing proficiency of interpretation of rapid human immunodeficiency virus assays in nonlaboratory settings: Ensuring quality of testing
    Learmonth, Kate M.
    McPhee, Dale A.
    Jardine, Darren K.
    Walker, Sandy K.
    Aye, Thein-Thein
    Dax, Elizabeth M.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (05) : 1692 - 1697
  • [10] Validation, Performance under Field Conditions, and Cost-Effectiveness of Capillus HIV-1/HIV-2 and Determine HIV-1/2 Rapid Human Immunodeficiency Virus Antibody Assays Using Sequential and Parallel Testing Algorithms in Tanzania
    Mayhood, Meghan K.
    Afwamba, Isaac A.
    Odhiambo, Christopher O.
    Ndanu, Epimack
    Thielman, Nathan M.
    Morrissey, Anne B.
    Shao, John F.
    Pence, Brian Wells
    Crump, John A.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (12) : 3946 - 3951