Fractionation of carbon (C-13/C-12) isotopes in glycine decarboxylase reaction

Fractionation of carbon isotopes (C-13/C-12) by glycine decarboxylase (GDC) was investigated in mitochondrial preparations isolated from photosynthetic tissues of different plants (Pisum, Medicago, Triticum, Hordeum, Spinacia, Brassica, Wolffia). 20 mM glycine was supplied to mitochondria, and the CO2 formed was absorbed and analyzed for isotopic content, CO2 evolved by mitochondria of Pisum was enriched up to 8 parts per thousand in C-12 compared to the carboxylic atom of glycine, CO2 evolved by mitochondria of the other plants investigated was enriched by 5-16 parts per thousand in C-13. Carbon isotope effects were sensitive to reaction conditions (pH and the presence of GDC cofactors), Theoretical treatment of the reaction mechanism enabled us to conclude that the value and even the sign of the carbon isotope effect in glycine decarboxylation depend on the contribution of the enzyme-substrate binding step and of the decarboxylation step itself to the overall reaction rate, Therefore, the fractionation of carbon isotopes in GDC reaction was revealed which provides essential isotopic effects in plants in addition to the well-known effect of carbon isotope fractionation by the central photosynthetic enzyme, ribulose-1,5-biphosphate carboxylase.