Cell Shape and Substrate Rigidity Both Regulate Cell Stiffness

被引:317
作者
Tee, Shang-You [1 ]
Fu, Jianping [1 ,2 ]
Chen, Christopher S. [1 ,2 ]
Janmey, Paul A. [1 ,2 ,3 ,4 ]
机构
[1] Univ Penn, Inst Med & Engn, Philadelphia, PA 19104 USA
[2] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA
[3] Univ Penn, Dept Phys, Philadelphia, PA 19104 USA
[4] Univ Penn, Dept Physiol, Philadelphia, PA 19104 USA
基金
美国国家卫生研究院;
关键词
FORCE;
D O I
10.1016/j.bpj.2010.12.3744
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Cells from many different tissues sense the stiffness and spatial patterning of their microenvironment to modulate their shape and cortical stiffness. It is currently unknown how substrate stiffness, cell shape, and cell stiffness modulate or interact with one another. Here, we use microcontact printing and microfabricated arrays of elastomeric posts to independently and simultaneously control cell shape and substrate stiffness. Our experiments show that cell cortical stiffness increases as a function of both substrate stiffness and spread area. For soft substrates, the influence of substrate stiffness on cell cortical. stiffness is more prominent than that of cell shape, since increasing adherent area does not lead to cell stiffening. On the other hand, for cells constrained to a small area, cell shape effects are more dominant than substrate stiffness, since increasing substrate stiffness no longer affects cell stiffness. These results suggest that cell size and substrate stiffness can interact in a complex fashion to either enhance or antagonize each other's effect on cell morphology and mechanics.
引用
收藏
页码:L25 / L27
页数:3
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