Synaptotagmin-1 is required for fibroblast growth factor-1 release

被引:64
作者
LaVallee, TM
Tarantini, F
Gamble, S
Carreira, CM
Jackson, A
Maciag, T
机构
[1] Maine Med Ctr, Res Inst, Ctr Mol Med, Portland, ME 04106 USA
[2] Amer Red Cross, Holland Lab, Dept Mol Biol, Rockville, MD 20855 USA
关键词
D O I
10.1074/jbc.273.35.22217
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
By using p65 synaptotagmin-1 and fibroblast growth factor (FGF)-1:beta-galactosidase (beta-gal) NIH 3T3 cell cotransfectants, we demonstrate that a proteolytic fragment consisting of the extravesicular domain of synaptotagmin-1 is released into the extracellular compartment in response to temperature stress with similar kinetics and pharmacological properties as FGF-1:beta-gal, Using a deletion mutant that lacks 95 amino acids from the extravesicular domain of synaptotagmin-1, neither synaptotagmin-1 nor FGF-1:beta-gal are able to access the stress-induced release pathway. Furthermore, the p40 extravesicular fragment of synaptotagmin-1 is constitutively released in p40 synaptotagmin-1 NIH 3T3 cell transfectants, and this release is potentiated when the cells are subjected to temperature stress. These data demonstrate that the p40 fragment derived from synaptotagmin-1 is able to utilize the FGF-1 non-classical exocytotic pathway and that the release of FGF-1 is dependent on synaptotagmin-1.
引用
收藏
页码:22217 / 22223
页数:7
相关论文
共 42 条
[1]  
BENNETT MK, 1994, ANNU REV BIOCHEM, V63, P63, DOI 10.1146/annurev.biochem.63.1.63
[2]  
BENNETT MK, 1993, J NEUROSCI, V13, P1701
[3]  
Bergmeyer H.-U., 1965, Methods of Enzymatic Analysis, P736
[4]   MAMMALIAN HOMOLOGS OF CAENORHABDITIS-ELEGANS UNC-13 GENE DEFINE NOVEL FAMILY OF C-2-DOMAIN PROTEINS [J].
BROSE, N ;
HOFMANN, K ;
HATA, Y ;
SUDHOF, TC .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (42) :25273-25280
[5]  
BURGESS WH, 1985, J BIOL CHEM, V260, P1389
[6]   THE HEPARIN-BINDING (FIBROBLAST) GROWTH-FACTOR FAMILY OF PROTEINS [J].
BURGESS, WH ;
MACIAG, T .
ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :575-606
[7]   S100A13 is involved in the regulation of fibroblast growth factor-1 and p40 synaptotagmin-1 release in vitro [J].
Carreira, CM ;
LaVallee, TM ;
Tarantini, F ;
Jackson, A ;
Lathrop, JT ;
Hampton, B ;
Burgess, WH ;
Maciag, T .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (35) :22224-22231
[8]   A novel function for the second C2 domain of synaptotagmin - Ca2+-triggered dimerization [J].
Chapman, ER ;
An, S ;
Edwardson, JM ;
Jahn, R .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (10) :5844-5849
[9]   Fatty acylation of synaptotagmin in PC12 cells and synaptosomes [J].
Chapman, ER ;
Blasi, J ;
An, S ;
Brose, N ;
Johnston, PA ;
Sudhof, TC ;
Jahn, R .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 225 (01) :326-332
[10]   Direct interaction of a Ca2+-binding loop of synaptotagmin with lipid bilayers [J].
Chapman, ER ;
Davis, AF .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (22) :13995-14001