A Comparative Study of Genetic Diversity in Chickpea Based Upon Touchdown and Non-touchdown PCR Using ISSR Markers

被引：0
|
作者：
Kumar, Mahesh [1 ]
Chhokar, Vinod [1 ]
Kumar, Anil [1 ]
Sarla [2 ]
Beniwal, Vikas [3 ]
Aggarwal, Himanshu [3 ]
机构：
[1] Guru Jambheshwar Univ Sci & Technol, Dept Bio & Nano Technol, Hisar 125001, Haryana, India
[2] Govt PG Coll, Dept Biotechnol, Karnal 132001, Haryana, India
[3] Maharishi Markandeshwar Univ, Dept Biotechnol, Mullana 133207, Haryana, India
来源：
CHIANG MAI JOURNAL OF SCIENCE | 2015年 / 42卷 / 01期
关键词：
chickpea; genetic diversity; ISSR; PCR; CICER-ARIETINUM-L; GENUS CICER; DNA; POLYMORPHISMS; CULTIVARS; REPEAT; RAPD;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Chickpea is one of the world's most important but less studied leguminous food crops. In the present study, inter simple sequence repeat (ISSR) marker were employed to investigate genetic diversity of chickpea. Eighteen cultivars of chickpea were studied using six ISSR markers out of these three markers (GTG)(5), (GTGC)(4) and (TCC)(5) were found to generate reproducible DNA fragments. On an average 3 or more bands ranging from 0.25-3.0 kb were obtained in case of non touchdown PCR while in case of touchdown PCR 2 bands ranging from 0.25-4.0 kb were obtained. The cophenetic correlation coefficient (r) for non touchdown and touchdown PCR was found to be 0.79 and 0.9 respectively. For the selection of good parental material in breeding programs, the genetic data produced through the present study can be used to correlate with the relationships between morphological traits.
引用
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页码：117 / 125
页数：9
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