A Comparative Study of Genetic Diversity in Chickpea Based Upon Touchdown and Non-touchdown PCR Using ISSR Markers

被引：0

作者：

Kumar, Mahesh ^{[1
]}

Chhokar, Vinod ^{[1
]}

Kumar, Anil ^{[1
]}

Sarla ^{[2
]}

Beniwal, Vikas ^{[3
]}

Aggarwal, Himanshu ^{[3
]}

机构：

[1] Guru Jambheshwar Univ Sci & Technol, Dept Bio & Nano Technol, Hisar 125001, Haryana, India

[2] Govt PG Coll, Dept Biotechnol, Karnal 132001, Haryana, India

[3] Maharishi Markandeshwar Univ, Dept Biotechnol, Mullana 133207, Haryana, India

来源：

CHIANG MAI JOURNAL OF SCIENCE | 2015年 / 42卷 / 01期

关键词：

chickpea; genetic diversity; ISSR; PCR; CICER-ARIETINUM-L; GENUS CICER; DNA; POLYMORPHISMS; CULTIVARS; REPEAT; RAPD;

D O I：

暂无

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chickpea is one of the world's most important but less studied leguminous food crops. In the present study, inter simple sequence repeat (ISSR) marker were employed to investigate genetic diversity of chickpea. Eighteen cultivars of chickpea were studied using six ISSR markers out of these three markers (GTG)(5), (GTGC)(4) and (TCC)(5) were found to generate reproducible DNA fragments. On an average 3 or more bands ranging from 0.25-3.0 kb were obtained in case of non touchdown PCR while in case of touchdown PCR 2 bands ranging from 0.25-4.0 kb were obtained. The cophenetic correlation coefficient (r) for non touchdown and touchdown PCR was found to be 0.79 and 0.9 respectively. For the selection of good parental material in breeding programs, the genetic data produced through the present study can be used to correlate with the relationships between morphological traits.

引用

页码：117 / 125

页数：9

共 50 条

[21] Comparative study and genetic diversity in Malva using srap molecular markers
Qadir, Syamand Ahmed
Meerza, Chnar Hama Noori
Faraj, Aryan Mahmood
Hamafaraj, Kawa Khwarahm
Tobakari, Sherzad Rasul Abdal- La
Hamarashid, Sahar Hussein
CARYOLOGIA, 2022, 75 (03) : 101 - 108
[22] Genetic diversity of Monilinia laxa isolates from Hungary using PCR-based ISSR technique
Holb, I. J.
PHYTOPATHOLOGY, 2013, 103 (06) : 61 - 61
[23] Genetic diversity study in papaya (Carica papaya L.) cultivars using RAPD and ISSR markers
Sabara, Pritesh
Vakharia, Dinesh
INDIAN JOURNAL OF BIOTECHNOLOGY, 2018, 17 (01): : 101 - 109
[24] Genetic diversity study of Brassica campestris L. ssp chinensis Makino based on ISSR markers
Wang, Aihua
Zhou, Guolin
Lin, Chufa
Wang, Bingcai
Huang, Xingxue
deng, Yaohua
Zhang, Ruihua
CARYOLOGIA, 2017, 70 (01) : 48 - 54
[25] Study on genetic diversity between Origanum L. species based on genome size and ISSR markers
Jedrzejczyk, Iwona
INDUSTRIAL CROPS AND PRODUCTS, 2018, 126 : 201 - 207
[26] Comparative Analysis of Genetic Diversity among Bt Cotton Genotypes Using EST-SSR, ISSR and Morphological Markers
Ashraf, J.
Malik, W.
Iqbal, M. Z.
Khan, A. Ali
Qayyum, A.
Noor, E.
Abid, M. A.
Cheema, H. M. Naseer
Ahmad, M. Q.
JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY, 2016, 18 (02): : 517 - 531
[27] Genetic Diversity Analysis of Brassica Species Using PCR-Based SSR Markers
Raza, Ali
Mehmood, Sundas Saher
Ashraf, Farwa
Khan, Rao Sohail Ahmad
GESUNDE PFLANZEN, 2019, 71 (01): : 1 - 7
[28] A comparative analysis of the genetic diversity between inbred lines of Zinnia elegans using morphological traits and RAPD and ISSR markers
Ye, Y. M.
Zhang, J. W.
Ning, G. G.
Bao, M. Z.
SCIENTIA HORTICULTURAE, 2008, 118 (01) : 1 - 7
[29] Comparative Analysis of Genetic Diversity in Some Non-commercial Cultivars of Musa L. from Assam, India, Using Morphometric and ISSR Markers
Borborah, Kongkona
Saikia, Debanjali
Rehman, Mehzabin
Islam, Md Aminul
Mahanta, Saurov
Chutia, Jnandabhiram
Borthakur, S. K.
Tanti, Bhaben
INTERNATIONAL JOURNAL OF FRUIT SCIENCE, 2020, 20 : 1814 - 1828
[30] Detection of genetic variation and Fusarium resistance in turfgrass genotypes using PCR-based markers (ISSR and SCAR)
Al-Humaid, A.
Motawei, M. I.
Abdalla, M. Y.
Mana, F.
JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT, 2004, 2 (3-4): : 225 - 229

← 1 2 3 4 5 →