Development of SCAR markers and PCR assays for single or simultaneous species-specific detection of Phytophthora nicotianae and Pythium helicoides in ebb-and-flow irrigated kalanchoe

被引：7

作者：

Ahonsi, Monday O. ^{[1
]}

Ling, Yin ^{[1
]}

Kageyama, Koji ^{[1
]}

机构：

[1] Gifu Univ, River Basin Res, Gifu 5011193, Japan

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2010年 / 83卷 / 02期

基金：

日本学术振兴会;

关键词：

Multiplex PCR; Oomycetes; Recycled irrigation; SCAR markers; Sustainable disease management strategies; POLYMERASE-CHAIN-REACTION; SEQUENCE; REGION; PRIMERS;

D O I：

10.1016/j.mimet.2010.08.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phytophthora nicotianae and Pythium helicoides are important water-borne oomycete pathogens of irrigated ornamentals particularly ebb-and-flow irrigated kalanchoe in Japan. We developed novel PCR-based sequence characterized amplified region markers and assays for rapid identification and species-specific detection of both pathogens in separate PCR reactions or simultaneously in a duplex PCR. Published by Elsevier By.

引用

收藏

页码：260 / 265

页数：6

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