Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells

被引：13

作者：

Bialkowska, Kamila ^{[1
,2
]}

Milowska, Katarzyna ^{[1
]}

Michlewska, Sylwia ^{[3
]}

Sokolowska, Paulina ^{[2
,4
]}

Komorowski, Piotr ^{[2
,5
]}

Lozano-Cruz, Tania ^{[6
,7
]}

Gomez-Ramirez, Rafael ^{[6
,7
]}

de la Mata, Francisco Javier ^{[6
,7
]}

Bryszewska, Maria ^{[1
]}

机构：

[1] Univ Lodz, Fac Biol & Environm Protect, Dept Gen Biophys, 141-143 Pomorska St, PL-90236 Lodz, Poland

[2] Bionanopark Ldt, Mol & Nanostruct Biophys Lab, 114-116 Dubois St, PL-93465 Lodz, Poland

[3] Univ Lodz, Fac Biol & Environm Protect, Lab Microscop Imaging & Specialized Biol Tech, Banacha12-16, PL-90237 Lodz, Poland

[4] Med Univ Lodz, Dept Pharmacol & Toxicol, Zeligowskiego St 7-9, PL-90752 Lodz, Poland

[5] Lodz Univ Technol, Inst Mat Sci, Dept Biophys, 1-15 Stefanowskiego St, PL-90924 Lodz, Poland

[6] Univ Alcala, IQAR, Dept Organ & Inorgan Chem, Madrid 28805, Spain

[7] Networking Res Ctr Bioengn Biomat & Nanomed CIBER, Madrid 28029, Spain

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2021年 / 22卷 / 13期

关键词：

siRNA; carbosilane dendrimers; dendriplexes; nanocarriers; PAMAM DENDRIMERS; DELIVERY; TOOL; THERAPEUTICS; TRANSFECTION; EFFICIENCY; STABILITY; COCKTAILS; TOXICITY; BARRIERS;

D O I：

10.3390/ijms22137097

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of siRNA in gene therapy is mainly limited because of the problems with its transport into cells. Utilization of cationic dendrimers as siRNA carriers seems to be a promising solution in overcoming these issues, due to their positive charge and ability to penetrate cell membranes. The following two types of carbosilane dendrimers were examined: CBD-1 and CBD-2. Dendrimers were complexed with pro-apoptotic siRNA (Mcl-1 and Bcl-2) and the complexes were characterized by measuring their zeta potential, circular dichroism and fluorescence of ethidium bromide associated with dendrimers. CBD-2/siRNA complexes were also examined by agarose gel electrophoresis. Both dendrimers form complexes with siRNA. Moreover, the cellular uptake and influence on the cell viability of the dendrimers and dendriplexes were evaluated using microscopic methods and XTT assay on MCF-7 cells. Microscopy showed that both dendrimers can transport siRNA into cells; however, a cytotoxicity assay showed differences in the toxicity of these dendrimers.

引用

页数：15

共 46 条

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