Qualitative and quantitative analysis of retinol, retinyl esters, tocopherols and selected carotenoids out of various internal organs from different species by HPLC

被引：20

作者：

Schaeffer, Michael W. ^{[1
]}

Roy, Somdutta Sinha ^{[1
]}

Mukherjee, Shyamali ^{[1
]}

Nohr, Donatus ^{[2
]}

Wolter, Michael ^{[2
]}

Biesalski, Hans K. ^{[2
]}

Ong, David E. ^{[3
]}

Das, Sail K. ^{[1
]}

机构：

[1] Meharry Med Coll, Dept Biochem & Canc Biol, Nashville, TN 37208 USA

[2] Univ Hohenheim, Dept Biol Chem & Nutr, D-70593 Stuttgart, Germany

[3] Vanderbilt Univ Sch Med, Dept Biochem, Nashville, TN 37232 USA

来源：

ANALYTICAL METHODS | 2010年 / 2卷 / 09期

关键词：

PERFORMANCE LIQUID-CHROMATOGRAPHY; VITAMIN-A SUPPLEMENTATION; GUINEA-PIG; ALPHA-TOCOPHEROL; BETA-CAROTENE; ACID; PHASE; PALMITATE; IDENTIFICATION; DEFICIENCY;

D O I：

10.1039/c0ay00288g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report the validation of a reversed-phase gradient HPLC method allowing simultaneous quantification of retinol, retinyl esters, tocopherols and selected carotenoids in lung, liver and plasma of mouse, rat and guinea pig (gp) using a diode array detector. A significant species difference was observed regarding the distribution of retinol and retinyl esters. The levels of total retinol in lung, liver and plasma were in the following order: mouse >> rat > gp; rat > mouse > gp; and gp >> rat > mouse, respectively. Furthermore, comparison studies revealed similarities between the vitamin A profiles of human and gp lung samples.

引用

页码：1320 / 1332

页数：13

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